rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: Role of Nck adaptors in the regulation of endothelial cell-cell adhesion
  name:
    name part: Bray, Kristyn.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2011
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: The establishment of endothelial cell-cell contacts is critical for the development and maintenance of the vascular network. Endothelial cell adherens junctions (AJ) are cell-cell adhesion complexes consisting of clusters of vascular endothelial (VE) cadherin linked to the cortical actin cytoskeleton through catenins. Actin polymerization and remodeling are important for the formation, maturation, and turnover of AJ. Research was conducted to elucidate the role of the Nck family of SH2/SH3 domain-containing adaptor proteins, well established regulators of the actin cytoskeleton, in the formation and remodeling of AJ in endothelial cells. I tested the hypothesis that Nck adaptors contribute to the formation of a polarized vascular lumen through the enhancement of VE-cadherin-mediated cell-cell adhesions. The purpose of this study was to determine the role of Nck in the formation of VE-cadherin-based cell adhesions. To these aims, I used a combination of molecular genetics, cell biology, and high resolution fluorescent microscopy. In the experiment, the effects of calcium were seen in the development of cell-cell junctions. When calcium was removed with EGTA, the VE-cadherin was delocalized from the edges of the cells. The EGTA also disrupted the actin fibers. The reintroduction of calcium reestablished the endothelial junctions between cells.
  note: by Kristyn Bray.
  note: "Major Subject: Biomedical Sciences"
  note: Title from author supplied metadata (automated record created 2011-08-09 15:12:36).
  note: Electronic resource.
  note: Bachelor of Science Texas A&M University 2011 http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9661
  note: Includes bibliographical references.
  note: Text (Undergraduate thesis)
  subject:
    topic: Major Biomedical Sciences
  subject:
    topic: Actin cytoskeleton
  subject:
    topic: Endothelial cell-cell contacts
  subject:
    topic: Adherens junctions
  subject:
    topic: Catenins
  subject:
    topic: Vascular network
  subject:
    topic: Nck
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2011-05-9661
  record info:
    record content source: TXA
    record creation date: 110817
    record change date: 20151202142303.0
    record identifier: in00002707350