rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: New insights into the role of equine infectious anemia virus s2 protein in disease expression
  name:
    name part: Covaleda Salas, Lina M.
    role:
      role term: creator
  name:
    name part: Payne, Susan
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2011
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: Equine infectious anemia virus (EIAV) is an important animal model to study the contribution of macrophages in viral persistence during lentiviral infections. EIAV is unique amongst the lentiviruses in that it causes a rapid, rather than the very slow disease progression, characteristic of other lentiviral infections. The accessory gene, S2, unique to EIAV, is an important determinant in viral pathogenesis. A functional S2 gene is required to achieve high-titer viremia and the development of disease in infected horses. Despite its essential role, the mechanisms by which S2 influences EIAV pathogenesis remain elusive. The goal of this research was to gain insight into the role of S2 in pathogenesis. To accomplish this goal we: (i) Examined the effects of EIAV and its S2 protein in the regulation of the cytokine and chemokine responses in macrophages, (ii) Assessed the influence of EIAV infection and the effect of S2 on global gene expression in macrophages and (iii) Identified host cellular proteins that interact with S2 as a starting point for the identification of host factors implicated in S2 function. The results from this study provide evidence for a role of S2 in enhancing a proinflammatory cytokine and chemokine response in infected macrophages. Specifically, S2 enhances the expression of IL-1 alpha, IL-1 beta IL-8, MCP-2, MIP-1 beta, IP-10 and a newly discovered cytokine, IL-34. Involvement of S2 in cytokine and chemokine dysregulation may contribute to disease development by optimizing the host cell environment to promote viral dissemination and replication. Microarray analyses revealed an interesting set of differentially expressed genes upon EIAV infection. Genes affected by EIAV were involved in the immune response, transcription, translation, cell cycle and cell survival. Finally, we used the yeast two-hybrid system to identify S2 host cellular interacting proteins. We identified osteosarcoma amplified 9 (OS-9) and proteasome 26S ATPase subunit 3 (PSMC3)…
  note: by Lina Maria Covaleda Salas.
  note: "Major Subject: Veterinary Microbiology"
  note: Title from author supplied metadata (automated record created 2011-08-09 15:09:44).
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2010 http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7975
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major veterinary microbiology
  subject:
    topic: Real-time PCR
  subject:
    topic: Gene expression
  subject:
    topic: Cytokines
  subject:
    topic: Macrophage
  subject:
    topic: EIAV
  subject:
    topic: Microarray
  subject:
    topic: Yeast two-hybrid system
  subject:
    topic: Chemokines
  subject:
    topic: Dysregulation
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7975
  record info:
    record content source: TXA
    record creation date: 110817
    record change date: 20151202142301.0
    record identifier: in00002707340