http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
phenotypic evaluation of 61 mutated lines of TAM 94L-25
Among the available methods of creating selectable variation, induced mutagenesis has been historically under-utilized in cotton improvement. Dick Auld showed that chemical mutagenesis could be used to enhance fiber length of a medium staple cotton cultivar without sacrificing yield. The goal of this project was to determine if mutagenesis could be used to improve the fiber quality of a germplasm line already considered to be at the upper-limits of fiber length. Seed of TAM 94 L-25 were treated with EMS in 2001 and the M₂ generation was produced at Lubbock, Texas in 2002. More than 1200 M₃ plants were grown at College Station, Texas in 2004, harvested individually, and HVI fiber properties determined. The top and bottom 1% for UHML, strength, and elongation were selected and the seeds of these individuals planted as an M₄ progeny row nursery in 2005. Approximately ten individual plants per progeny row were harvested for re-evaluation of fiber parameters. From the approximately 1600 individual TAM 94L-25 M₄ plants harvested in 2005, 61 were selected and subsequently treated as pure lines. Agronomic performance trials were conducted on 61 of those TAM 94L-25 M lines along with the M₀ check and two commercial cultivar checks, Fiber Max 832 and Phytogen 355, in 2008 and 2009 in College Station and Weslaco, Texas. Within-boll yield components were examined for 13 representative mutant lines and the three checks. TAM 94L-25 averaged 751 kg lint ha⁻¹, 31.1 mm UHML, 303 kN m kg⁻¹ fiber bundle strength, and 6.0 percent elongation. The 61 mutant lines yielded from 366 to 932 kg lint ha⁻¹, exhibited UHML from 24.3 to 34.9 mm, fiber bundle strengths of 261 to 333 kN m kg⁻¹, and elongations from 5.4 to 8.1 percent. Seed surface area of the TAM 94L-25 M-lines was between 99 and 124 mm², and fibers per unit seed surface area from 123 to 168 fibers mm⁻². The M₀ parent, TAM 94L-25 averaged 125 mm² seed⁻¹, and 128 fibers mm⁻². The data presented herein demonstrate that EMS-induced mutagenesis was successful in creating TAM 94L-25 M-lines with superior fiber and yield traits to that of the non-mutated, high fiber quality parent, TAM 94L-25.
Title from author supplied metadata (automated record created 2011-02-28 11:02:00).
Master of Science Texas A&M University 2010 http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8898