Preparative-scale isoelectric trapping separations in a multicompartmental electrolyzer : implementation and monitoring /
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[College Station, Tex.] :
[Texas A&M University],
[2010]
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| Online Access: | Link to OAK Trust copy |
| Abstract: | Preparative-scale protein separations have always been critical to the advancement of the life sciences. Among preparative-scale separation techniques, isoelectric trapping (IET) promises efficient separations and high production rates. This dissertation focuses on the improvement of two aspects of preparative-scale IET protein separations: the instrumentation used and the monitoring of the separation. The first aspect (preparative-scale) is the IET device: the improvement of a multicompartmental electrolyzer (MCE) to increase the efficiency and production rate of IET separations. The redesign focused on three major areas: (1) the sealing system, (2) the configuration of the liquid flow path, and (3) the cooling system. The second aspect (analytical-scale) is the monitoring of the IET separation: the design and manufacture of durable surface-modified capillaries which provide controlled, variable anodic and cathodic electroosmotic flow (EOF) to help develop, plan, and monitor the IET separations. |
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| Item Description: | "Major Subject: Chemistry" Title from author supplied metadata (automated record created 2010-03-12 12:08:51). Electronic resource. |
| Physical Description: | 1 online resource. |
| Bibliography: | Includes bibliographical references. |