rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Improved fluorescence-enhanced optical imaging and tomography by enhanced excitation light rejection
  name:
    name part: Hwang, Kil Dong.
    role:
      role term: creator
  name:
    name part: Sevick-Muraca, Eva
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2010
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: remote
    extent: 1 online resource.
  abstract: Fluorescence enhanced optical imaging and tomography studies involve the detection of weak fluorescent signals emanating from nano- to picomolar concentrations of exogenous or endogenously produced fluorophore concurrent with the rejection of an overwhelmingly large component of backscattered excitation light. The elimination of the back-reflected excitation light of the collected signal remains a major and often unrecognized challenge for further reducing the noise floor and increasing sensitivity of small animal fluorescence imaging. In this dissertation, we adapted collimating and gradient index (GRIN) lenses in an existing frequency-domain system to improve excitation light rejection and enhance planar and tomographic imaging. To achieve this goal, we developed planar and tomographic imaging systems based upon ray tracing calculations for improved rejection of excitation light. The "out-of-band (S ([lambda subscript x]))" to "in-band (S ([lambda subscript m]) - S ([lambda subcript x]))" signal ratio assessing excitation leakage was acquired with and without collimating optics. The addition of collimating optics resulted in a 51 to 75% reduction in the transmission ratio of (S ([lambda subscript x]))/ (S ([lambda subscript m]) - S ([lambda subscript x])) for the phantom studies and an increase of target to background ratio (TBR) from 11% to 31% in animal studies. Additionally, we presented results demonstrating the improvement of model match between experiments and forward simulation models by adaptation of GRIN lens optics to a breast phantom study. In particular, 128 GRIN lenses on the fiber bundle face were employed to align the collected excitation and emission light normal to the filter surface in an existing frequency-domain system. As a result of GRIN lens collimation, we reduced the transmission ratio between 10 and 86 % and improved the model match for tomographic reconstruction of one (1 cm³) and two (0.1 cm³) targets in a 1087 cm³ of breast phantom. Ul…
  note: by Kil Dong Hwang.
  note: "Major Subject: Chemical Engineering"
  note: Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2006 http://hdl.handle.net/1969.1/ETD-TAMU-1062
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major chemical engineering
  subject:
    topic: IMAGING
  subject:
    topic: REJECTION
  subject:
    topic: EXCITATION
  subject:
    topic: FLUORESCENCE
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1062
  record info:
    record content source: TXA
    record creation date: 100429
    record change date: 20151201150216.0
    record identifier: in00002587241