rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Poly(vinyl alcohol)-based buffering membranes for isoelectric trapping separations
  name:
    name part: Craver, Helen C Fleisher.
    role:
      role term: creator
  name:
    name part: Vigh, Gyula
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2010
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: remote
    extent: 1 online resource.
  abstract: Isoelectric trapping (IET) in multicompartment electrolyzers (MCE) has been widely used for the electrophoretic separation of ampholytic compounds such as proteins. In IET, the separation occurs in the buffering membranes that form a step-wise pH gradient in the MCE. Typically, buffering membranes have been made by copolymerizing acrylamide with Immobiline compounds, which are acidic and basic acylamido buffers. One major problem, however, is that these buffering membranes are not stable when exposed to high concentrations of acid and base due to hydrolysis of the amide bonds. Poly(vinyl alcohol)-based, or PVA-based, membranes were made as an alternative to the polyacrylamide-based membranes since they provide more hydrolytic and mechanical stability. Four mid-pH, PVA-based buffering membranes that contain single ampholytes were synthesized. These buffering membranes were used to trap small molecular weight pI markers for up to three hours, and were also used in desalting experiments to remove strong electrolytes from a solution of ampholytes. Additionally, the membranes were used in IET experiments to separate mixtures of pI markers, and to fractionate the major proteins in chicken egg white. The membranes did not show any degradation when stored in 3 M NaOH for up to 6 months and were shown to tolerate current densities as high as 16 mA/cm². In addition, six series of PVA-based membranes, whose pH values can be tuned over the 3 < pH < 10 range, were synthesized by covalently binding aminodicarboxylic acids, and monoamines or diamines to the PVA matrix. These tunable buffering membranes were used in trapping experiments to trap ampholytes for up to three hours, and in desalting experiments to remove strong electrolytes from a solution of ampholytes. These tunable buffering membranes were also used in IET experiments to separate proteins, some with pI values that differ by only 0.1 pH unit. The tunable buffering membranes did not show any signs of degradation when e…
  note: by Helen C. Fleisher Craver.
  note: "Major Subject: Chemistry"
  note: Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2007 http://hdl.handle.net/1969.1/ETD-TAMU-1254
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major chemistry
  subject:
    topic: protein separations
  subject:
    topic: buffering membranes
  subject:
    topic: isoelectric trapping
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1254
  record info:
    record content source: TXA
    record creation date: 100429
    record change date: 20151201150206.0
    record identifier: in00002586702