rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Spectral multiplexing using quantum dot tagged microspheres with diffusing colloidal probe microscopy
  name:
    name part: Muthukumar, Shankarapandian.
    role:
      role term: creator
  name:
    name part: Meissner, Kenith E.
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2010
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: remote
    extent: 1 online resource.
  abstract: This work involves the development of a new technique that integrates Diffusing Colloidal Probe Microscopy (DCPM) and luminescence to simultaneously measure multiple particle-wall interactions. DCPM can be used to map potential energy profiles of multiple particle-surface interactions simultaneously and accurately. Colloidal semiconductor quantum dots were used for spectral multiplexing to enable monitoring of multiple analytes at the same time. DCPM combines Total Internal Reflection Microscopy (TIRM) and Video Microscopy to simultaneously measure multiple particle-surface interactions with nanometer resolution in particle-surface separation. By acquiring the scattered intensity emitted by the particles, the separation distance can be calculated and subsequently the forces of interactions between the particle and the surface. This work demonstrates the use of luminescence instead of scattering as the mode of detection in DCPM. The luminescence is provided by quantum dots which are incorporated into polystyrene microspheres. The unique optical properties of quantum dots enable the creation of an optically multiplexed system where microspheres are tagged by quantum dots of different emission wavelengths. Scattering in DCPM may result in erroneous calculation of the potential energy profiles because of particle polydispersity. Since scattering is dependent on particle size, luminescence is introduced into the system and some interesting results are obtained. These results illustrate that the effect of particle polydispersity is significantly reduced when luminescence is used as the mode of detection. This combined with the DCPM system's sensitivity would enable the monitoring of multiple functionalized particlesurface interactions simultaneously and accurately.
  note: by Shankarapandian Muthukumar.
  note: "Major Subject: Biomedical Engineering"
  note: Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
  note: Electronic resource.
  note: Master of Science Texas A&M University 2007 http://hdl.handle.net/1969.1/ETD-TAMU-1245
  note: Includes bibliographical references.
  note: Text (Thesis)
  subject:
    topic: Major biomedical engineering
  subject:
    topic: TIRM
  subject:
    topic: Quantum Dot
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1245
  record info:
    record content source: TXA
    record creation date: 100429
    record change date: 20151201150156.0
    record identifier: in00002586621