rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Mechanisms of coactivation of estrogen receptor alpha (ER alpha)- and ER alpha/Sp-mediated gene transactivation by vitamin D receptor interacting protein 205 (DRIP205) in breast cancer cells
  name:
    name part: Wu, Qian.
    role:
      role term: creator
  name:
    name part: Safe, Stephen H.
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2010
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: remote
    extent: 1 online resource.
  abstract: Vitamin D interacting protein 205 (DRIP205) is a mediator complex protein that anchors the complex to the estrogen receptor (ER) and other nuclear receptors (NRs). In ZR-75 breast cancer cells treated with 17β-estradiol (E2) and transfected with a construct containing three tandem estrogen responsive elements (pERE3), DRIP205 coactivates ERα-mediated transactivation. DRIP205Δ587-636 is a DRIP205 mutant in which both NR boxes within amino acids 587-636 have been deleted and, in parallel transfection studies, DRIP205Δ587-636 also coactivates ERα. Moreover, both wild-type and variant DRIP205 also colocalize with ERα in the nuclei of transfected cells. AF1 and AF2 of ERα are both required for DRIP205 coactivation. Extensive deletion analysis of DRIP205 shows that multiple domains of this protein play a role in coactivation of ERα and in interactions with ERα. On the other hand, both DRIP205 and DRIP205Δ587-636 coactivate E2-induced transactivation of ERα/Sp1 in cells transfected with a construct containing three GC-rich sites (pSp13). Coactivation of ERα/Sp1 by DRIP205 is dependent on AF1 of ERα. Enhancement of ERα and ERα/Sp1 by DRIP205 does not require NR boxes of DRIP205, and deletion mutants DRIP205 (1-714) and DRIP205 (516-1566) significantly coactivate ERα and ERα/Sp1. RNA interference study showed that DRIP205 coactivation of ERα/Sp was abolished in cells transfected with iSp3 and iSp4, suggesting that Sp3 and Sp4 are required for coactivation of ERα/Sp by DRIP205 in ZR-75 cells.
  note: by Qian Wu.
  note: "Major Subject: Biochemistry"
  note: Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2005 http://hdl.handle.net/1969.1/ETD-TAMU-1008
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major biochemistry
  subject:
    topic: estrogen receptor
  subject:
    topic: DRIP205
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1008
  record info:
    record content source: TXA
    record creation date: 100423
    record change date: 20151201120051.0
    record identifier: in00002578699