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00000cam a2200000Ka 4500 |
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20151201150152.0 |
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m f d |
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cr unu|||||||| |
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100428s2010 txu sbm 000 0 eng d |
| 035 |
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|a (OCoLC)ocn609905162
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| 035 |
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|a (OCoLC)609905162
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| 035 |
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|a (TxCM)http://hdl.handle.net/1969.1/ETD-TAMU-1701
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| 040 |
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|a TXA
|c TXA
|d UtOrBLW
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| 049 |
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|a TXAM
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| 099 |
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|a 2005
|a Dissertation
|a 1969.1/ETD-TAMU-1701
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| 100 |
1 |
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|a Chandler, Aglaia.
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| 245 |
1 |
4 |
|a The role of RpoT;3 in chloroplast development and gene expression /
|c by Aglaia Chandler.
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| 264 |
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1 |
|a [College Station, Tex.] :
|b [Texas A&M University],
|c [2010]
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| 300 |
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|a 1 online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 500 |
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|a "Major Subject: Biochemistry"
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| 500 |
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|a Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
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| 502 |
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|b Doctor of Philosophy
|c Texas A&M University
|d 2005
|o http://hdl.handle.net/1969.1/ETD-TAMU-1701
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| 504 |
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|a Includes bibliographical references.
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| 516 |
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|a Text (Dissertation)
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3 |
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|a The real-time PCR assay method was used to quantify the RNA abundance of twenty-eight plastid genes in a range of tissues and developmental stages of Arabidopsis thaliana. Three groups of co-regulated genes were identified. Three trn genes (Cluster I) showed differential expression in siliques. Genes encoding components of the plastid transcription and translation apparatus, the energetic apparatus as well as two genes encoding components of the plastid protease and acetyl-CoA carboxylase, showed maximum transcript accumulation at the 2-day stage (Cluster IIA and IIB). Finally, the genes encoding components of the photosynthetic apparatus of Cluster III reached maximum transcript abundance in later stages of chloroplast development. This coordinated expression of plastid genes reflects the presence of regulatory mechanisms that modulate plastid gene expression in different plant tissues and developmental stages. We identified an Arabidopsis mutant, rpoZ191, in which a T-DNA is inserted in the RpoT;3 gene encoding the plastid-targeted phage-type NEP enzyme. The mutant displays a general reduction in growth, as well as a delay in greening and in chloroplast development. Real-time PCR analysis of plastid RNA accumulation showed that the RpoT;3 mutation caused a significant decrease in plastid transcript accumulation at the 2-day stage and a smaller inhibition at the 5-day stage. No major effects of the RpoT;3 mutation on the accumulation of plastid transcripts was observed in mature seeds and 5-day roots. Additionally, plastid transcript accumulation in mutant siliques was not significantly different from the wild-type, except for trnfM-CAU and trnW-CCA, which showed enhanced transcript levels. Taken together, these data indicate that the RpoT;3 NEP enzyme plays an important role in the overall transcription of plastid genes during the early phases of chloroplast and leaf differentiation. Furthermore, a functional RpoT;3 is required for the activation of selected nucleus-encoded plastid-localized proteins. However, the enhanced activity of RpoT;3 during the early stages of chloroplast differentiation is not due to an increase in RpoT;3 mRNA abundance. We suggest that post-transcriptional mechanisms (e.g. phosphorylation, specificity factors) activate the transcription of plastid RpoT;3 - transcribed genes during the early stages of plastid development.
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| 500 |
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|a Electronic resource.
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| 650 |
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|a Major biochemistry.
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| 653 |
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|a NEP
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| 653 |
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|a chloroplast
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| 700 |
1 |
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|a Mullet, John E.,
|e thesis advisor.
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| 856 |
4 |
0 |
|u http://hdl.handle.net/1969.1/ETD-TAMU-1701
|z Link to OAK Trust copy
|t 0
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| 948 |
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|a cataloged
|b h
|c 2010/4/28
|d c
|e jgreene
|f 4:47:05 pm
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| 994 |
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|a C0
|b TXA
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| 999 |
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|a MARS
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| 999 |
f |
f |
|s 453ea77b-76bf-338e-aa4f-f656bd405fe7
|i c5df656c-12fb-34e6-b37a-f8c53389668e
|t 0
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| 952 |
f |
f |
|a Texas A&M University
|b College Station
|c Electronic Resources
|d Available Online
|t 0
|e 2005 Dissertation 1969.1/ETD-TAMU-1701
|h Other scheme
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| 998 |
f |
f |
|a 2005 Dissertation 1969.1/ETD-TAMU-1701
|t 0
|l Available Online
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