rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Effects of exercise or oocyte heat shock on embryo development and gene expression in the horse
  name:
    name part: Mortensen, Christopher John.
    role:
      role term: creator
  name:
    name part: Vogelsang, Martha M.
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 2010
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: electronic
    form: remote
    extent: 1 online resource.
  abstract: Horse owners commonly maintain their broodmares in training and competition during the breeding season. The effect this has on mare reproductive efficiency has received limited attention. Heat stress has shown to be detrimental to oocyte competence in other species and heat shock protein 70 has been shown to be an important gene in regulating cellular response to heat. Mares were exercised in a hot humid environment to determine the effects on reproductive efficiency. Embryos were collected at d 7 after ovulation from exercised and control mares. Oocyte developmental competence was measured after oocytes were subjected to a one time heat shock, 42 °C for 2 or 4 h, at the onset or near completion of in vitro maturation. Embryos from both previous experiments were examined for HSP70 gene expression by real time RT-PCR. Exercised mares ovulated significantly smaller follicles, 39.8 vs. 41.5 mm diameter, and ovulated later after being given PGF2[alpha], 8.5 vs. 9.2 d. Twenty-two embryos (22/35) were recovered from control mares, recovery rate of 63%. Significantly fewer embryos were recovered in exercised mares (11/32), recovery rate of 34%. A lower proportion of grade 1 embryos were recovered from exercised versus control mares (4/11 vs.16/22,respectively). No effect was observed on oocyte nuclear maturation or embryonic development after ICSI when oocytes were exposed to heat shock at the onset of IVM. A heat shock of 42 °C for 2 or 4 h on oocytes during late IVM resulted, however, in a significantly lower rate of nuclear maturation, and a significant decrease in advanced embryo development (morulae plus blastocysts). Heat shock protein 70 gene expression was shown to be related to quality score of in vivo-recovered embryos, with lower quality embryos recording a significantly higher relative expression. Heat shock of late stage IVM oocytes for 4 h resulted in significantly higher blastocyst HSP70 expression. Results of this study indicate that exercise in a hot humid…
  note: by Christopher John Mortensen.
  note: "Major Subject: Animal Science"
  note: Title from author supplied metadata (automated record created 2010-03-12 12:08:51).
  note: Electronic resource.
  note: Doctor of Philosophy Texas A&M University 2007 http://hdl.handle.net/1969.1/ETD-TAMU-1232
  note: Includes bibliographical references.
  note: Text (Dissertation)
  subject:
    topic: Major animal science
  subject:
    topic: Oocyte
  subject:
    topic: Horse
  subject:
    topic: Reproduction
  subject:
    topic: Embryo
  subject:
    topic: Mare
  subject:
    topic: Gene
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-1232
  record info:
    record content source: TXA
    record creation date: 100427
    record change date: 20151201120131.0
    record identifier: in00002571192