Cytochrome P450 protocols /
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[2006]
|
| Edition: | 2nd ed. |
| Series: | Methods in molecular biology (Clifton, N.J.) ;
320. |
| Subjects: | |
| Online Access: | Connect to the full text of this electronic book |
Table of Contents:
- Cytochrome P450 nomenclature, 2004
- Spectral analyses of cytochromes P450
- Establishment of functional human cytochrome P450 monooxygenase systems in Escherichia coli
- Purification of cytochromes P450: products of bacterial recombinant expression systems
- Expression of recombinant flavin-containing monooxygenases in a baculovirus/insect cell system
- Cytochrome P450 reconstitution systems
- Catalytic assays for human cytochrome P450: an introduction
- Enzymatic analysis of cDNA-expressed human CYP1A1, CYP1A2, and CYP1B1 with 7-ethoxyresorufin as substrate
- Spectrofluorometric analysis of CYP2A6-catalyzed coumarin 7-hydroxylation
- Determination of CYP2B6 component of 7-ethoxy-4-trifluoromethylcoumarin O-deethylation activity in human liver microsomes
- Determination of CYP4A11-catalyzed lauric acid 12-hydroxylation by high-performance liquid chromatography with radiometric detection
- An isocratic high-performance liquid chromatography assay for CYP7A1-catalyzed cholesterol 7[alpha][alpha]-hydroxylation
- Use of 7-ethoxycoumarin to monitor multiple enzymes in the human CYP1, CYP2, and CYP3 families
- Benzydamine N-oxygenation as a measure of flavin-containing monooxygenase activity
- Prochiral sulfoxidation as a probe for flavin-containing monooxygenases
- Targeting antipeptide antibodies toward cytochrome P450 enzymes
- The human cytochrome P450 allele nomenclature committee web site: submission criteria, procedures, and objectives
- Genotyping for cytochrome P450 polymorphisms
- Hepatocyte cultures in drug metabolism and toxicological research and testing
- Isolation of rat hepatocytes
- Rat hepatocyte cultures: conventional monolayer and cocultures with rat liver epithelial cells
- Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures
- Hepatocytes in suspension
- Isolation of rat bone marrow stem cells
- Transfection of primary cultures of rat hepatocytes
- Human hepatocyte culture
- Determination of cellular localization of the expression of flavin-containing monooxygenase genes in mouse tissues by In Situ hybridization
- Deletion of genes from the mouse genome using Cr/loxP technology
- Characterization of targeted mouse embryonic stem cell chromosomes: karyotyping and fluorescence in situ hybridization of metaphase spreads
- Microinjection of targeted embryonic stem cells and establishment of knockout mouse lines for Fmo genes
- Use of reporter genes to measure xenobiotic-mediated activation of CYP gene transcription.
- High-performance liquid chromatography analysis of CYP2C8-catalyzed paclitaxel 6[alpha] [alph]-hydroxylation
- Determination of CYP2C9-catalyzed diclofenac 4¹-hydroxylation by high-performance liquid chromatography
- CYP2C19-mediated (S)-mephenytoin 4¹-hydroxylation assayed by high-performance liquid chromatography with radiometric detection
- CYP2D6-dependent bufuralol 1¹-hydroxylation assayed by reverse-phase ion-pair high-performance liquid chromatography with fluorescence detection
- Spectrophotometric analysis of human CYP2E1-catalyzed p-nitrophenol hydroxylation
- Thin-layer chromatography analysis of human CYP3A-catalyzed testosterone 6[beta][beta]-hydroxylation