Table of Contents:
  • Breast tissue banking: collection, handling, storage, and release of tissue for breast cancer research / Linda Snell and Peter H. Watson
  • Frozen biopsy collection and storage / Russell D. Leek
  • Serial biopsies/FNAs and their assessment / Irene Boeddinghaus and Stephen R.D. Johnson
  • Breast tissue microarrays / Robert J. Springall and Cheryl E. Gillett
  • Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA / Naomi Robertson and Russell Leek
  • Isolation of RNA from tumor samples: single-step guanidinium acid/phenol method / Naomi Robertson and Russell Leek
  • Isolation of DNA from tumor samples / Naomi Robertson and Russell Leek
  • Laser-assisted microdissection and isolation of DNA and RNA / Ulrich Lehmann and Hans Kreipe
  • Fluorescence in situ hybridization and comparative genomic hybridization / Patricia Gorman and Rebecca Roylance
  • Fluorescence in situ hybridization assessment of c-myc amplification in breast cancer tissues / Jan K. Blancato, Mary Steele Williams, and Robert B. Dickson
  • Detection of HER2 gene amplification by FISH in breast cancer / John M.S. Bartlett, and Amanda Forsyth
  • In situ hybridization combined with immunohistochemistry to localize gene expression / Rosemary Jeffery, Toby Hunt, and Richard Poulsom
  • Quantitation of RNA by ribonuclease protection assay / John W. Moore
  • Identification of steroid hormone-regulated genes in breast cancer / Bruce R. Westley and Felicity E.B. May
  • Sequencing of the tumor suppressor gene TP 53 / Barbro Linderholm, Torbjorn Norberg, and Jonas Bergh
  • Expression profiling using cDNA microarrays / Chris Jones ... [et al.]
  • Gene expression analysis using filter cDNA microarrays / Peter Simpson ... [et al.]
  • Immunohistochemistry / Cheryl E. Gillett
  • Detection of aberrant glycosylation in breast cancer using lectin histochemistry / Tracey M. Carter and Susan A. Brooks
  • SDS-PAGE and western blotting to detect proteins and glycoproteins of interest in breast cancer research / Chloe Osborne and Susan A. Brooks
  • Breast cancer proteomics using two-dimensional electrophoresis / Miriam V. Dwek and Sarah L. Rawlings
  • Procedures for the quantitative protein determination of urokinase and its inhibitor PAI-1 in human breast cancer tissue extracts using ELISA / Manfred Schmitt ... [et al.]
  • Methods to analyze the effects of the urokinase system on cancer cell adhesion, proliferation, migration, and signal transduction events / Ute Reuning ... [et al.]
  • Phosphospecific antibodies as a tool to study in vivo regulation of BRCA1 after DNA damage / Kum Kum Khanna, Magtouf Gatei, and Gordon Tribbick
  • Models of hormone resistance in vitro and in vivo / Jennifer MacGregor Schafer and V. Craig Jordan
  • Generation of genetically modified embryonic stem cells for the development of knockout mouse animal model systems / Stephen D. Robinson, Stephen Wilson, and Kairbaan M. Hodivala-Dilke
  • In vivo xenograft models of breast cancer metastatsis / Ursula Valentiner, Susan A. Brooks, and Udo Schumacher
  • Neoadjuvant endocrine therapy models / Juliette Murray, William R. Miller, and J. Michael Dixon
  • Primary mouse endothelial cell culture for assays of angiogenesis / Louise E. Reynolds and Kairbaan M. Hodivala-Dilke.
  • Traditional and established indicators of prognosis and treatment success / Derek E. Roskell and Ian D. Buley
  • Sentinel lymph node biopsy in breast cancer / Celia Chao and Kelly M. McMasters
  • Pathological evaluation of axillary sentinel lymph nodes in breast cancer / Michael Z. Gilcrease and Aysegul Sahin
  • Measurement of estrogen receptor status by immunocytochemistry in paraffin wax sections / Bharat Jasani ... [et al.]
  • Markers of apoptosis / Falzul H. Sarkar and Yiwei Li
  • Quantitative angiogenesis in breast cancer / Stephen B. Fox