Regulation of cloned cardiac channels /
| Main Author: | |
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| Other Authors: | , |
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[College Station, Tex.] :
[Texas A&M University],
[2005]
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| Subjects: | |
| Online Access: | Link to OAK Trust copy |
| Abstract: | Activation of a₅[Beta]₁ integrin potentiates L-type calcium current in vascular smooth muscle, which is partly mediated by tyrosine phoshorylation of the a1c channel subunit.Expressed rabbit VSM and neuronal isoforms are also potentiated by a₅[Beta]₁ integrin activation and require dual phosphorylation of a1c by PKA and c-Src. To explore common mechanisms of regulation by a₅[Beta]₁ integrin, whole cell patch clamp experiments were used to investigate the effects of a₅[Beta]₁ integrin antibody on expressed cardiac calcium channels. In HEK cells transfected with a1c, [Beta]2a and a2-d1 subunits alone, currents increased 1.8 - 2.0 fold on application of a₅[Beta]₁ antibody. The potentiation was almost completely abolished on the application of PKI, a highly specific Protein Kinase A (PKA) inhibitor. The expressed currents increased 2.0 - 2.2 fold on application of PKA activator 8-Br-cAMP, and abolished by PKI. Our results suggest that regulation of L-type calcium channels by a₅[Beta]₁ integrin is a general mechanism shared by VSM, neuronal and cardiac channels. However, in the cardiac isoform, only PKA phosphorylation is involved. |
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| Item Description: | "Major Subject: Biomedical Engineering" Title from author supplied metadata (automated record created on Nov. , 09:45:50.) Vita. Abstract. Electronic resource. |
| Physical Description: | 1 online resource. |
| Format: | System requirements: Adobe Acrobat Reader. |
| Bibliography: | Includes bibliographical references. |