Mechanistic investigations of the A-cluster of acetyl-CoA synthase /
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| Other Authors: | |
| Format: | Thesis eBook |
| Language: | English |
| Published: |
[College Station, Tex.] :
[Texas A&M University],
[2006]
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| Subjects: | |
| Online Access: | Link to OAK Trust copy |
| Abstract: | The A-cluster of acetyl-CoA synthase (ACS) catalyzes the formation of acetyl-CoA from CO, coenzyme-A, and a methyl group donated by a corrinoid iron-sulfur protein. Recent crystal structures have exhibited three different metals, Zn, Cu, and Ni, in the proximal site, which bridges a square-planar nickel site and a [Fe4S4] cubane. Contradicting reports supported both the nickel and copper containing forms as representing active enzyme. The results presented here indicate that copper is not necessary or sufficient for catalysis and that copper addition to ACS is deleterious. Several proposed mechanisms exist for the synthesis of acetyl-CoA, the two most prominent are the 'paramagnetic' and 'diamagnetic' mechanisms. The 'diamagnetic' mechanism proposes a two electron activation that precedes methylation to produce an EPR silent Ni²⁺-CH₃ species. This then reacts with CO and coenzyme-A to form acetyl-CoA and regenerate the starting species. The 'paramagnetic' mechanism assumes a one electron activation prior to the methylation of the paramagnetic Ni¹⁺-CO state to form anunstable Ni³⁺-acetyl species. This is immediately reduced by an electron shuttle. Results are presented here that no shuttle or external redox mediator is necessary for catalysis. This supports the 'diamagnetic' mechanism, specifically that a two-electron reductive activation is necessary and that the Ni¹⁺-CO species is not an intermediate.The two-electron reductive activation required by the 'diamagnetic' mechanism results in an unknown electronic state. Two proposals have been made to describe this form of the A-cluster. The first hypothesis from Brunold et al involves a one-electron reduction of the [Fe₄S₄] cube and a one-electron reduction of the Nip²⁺. This should result in a spin-coupled state that is S = integer. The Ni⁰ hypothesis requires both electrons to localize on the Nip²⁺ forming a zero-valent proximal nickel. Mössbauer spectroscopy has been used to probe the oxidation state and spin state of the [Fe₄S₄] cube in the reduced active form. No integer spin system is found and this is interpreted as supporting the Ni⁰ hypothesis. Additionally, spectra are presented that indicate the heterogeneous nature of the A-cluster is not caused by the occupancy of the proximal site. |
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| Item Description: | "Major Subject: Biochemistry" Title from author supplied metadata (automated record created on Apr. 14, 2006.) Vita. Abstract. Electronic resource. |
| Format: | Mode of access: World Wide Web. System requirements: World Wide Web access and Adobe Acrobat Reader. |
| Bibliography: | Includes bibliographical references. |