Practical skills in biomolecular sciences /

Bibliographic Details
Other Authors:	Reed, Robert (Robert H.)
Format:	Book
Language:	English
Published:	Harlow, England ; New York : Pearson/Prentice Hall, 2003.
Edition:	2nd ed.
Subjects:	Molecular biology > Laboratory manuals.
Online Access:	Table of contents

Table of Contents:

Machine generated contents note: List of Boxes vii
Preface to the Second Edition ix
For the Student xi
Acknowledgements xii
List of Abbreviations xiii
Study and examination skills
1 The importance of transferable skills 3
2 Managing your time 7
3 Working with others 12
4 Taking notes from lectures and texts 16
5 Revising 21
6 Assessments and exams 25
7 Preparing your curriculum vitae 33
Information technology and library resources
8 Finding and citing published information 41
9 Evaluating information 47
10 Using the Internet and World Wide Web 53
11 Bioinformatics - Internet resources 61
12 Using spreadsheets 68
13 Word processors, databases and other packages 74
Communicating information
14 Organising a poster display 83
15 Giving a spoken presentation 87
16 General aspects of scientific writing 93
17 Writing essays 99
18 Reporting practical and project work 103
19 Writing literature surveys and reviews 108
Fundamental laboratory techniques
20 Your approach to practical work 113
21 Health and safety 117
22 Working with liquids 121
23 Basic laboratory procedures 128
24 Principles of solution chemistry 138
25 pH and buffer solutions 146
26 Introduction to microscopy 154
27 Setting up and using a light microscope 159
The investigative approach
28 Making and recording measurements 169
29 SI units and their use 176
30 Scientific method and design of experiments 182
31 Project work 191
Handling cells and tissues
32 Sterile technique and microbial culture 197
33 Isolating and identifying microbes 205
34 Working with animal and plant tissues and cells 218
35 Culture systems and growth measurement 225
36 Homogenisation and fractionation of cells and tissues 237
Analytical techniques
37 Calibration and its application to quantitative analysis 245
38 Immunological methods 250
39 Radioactive isotopes and their uses 258
40 Light measurement 267
41 Basic spectroscopy 271
42 Advanced spectroscopy and spectrometry 280
43 Centrifugation 289
44 Chromatography - separation methods 296
45 Chromatography - detection and analysis 307
46 Principles and practice of electrophoresis 313
47 Advanced electrophoretic techniques 324
48 Electroanalytical techniques 331
Assaying biomolecules and studying metabolism
49 Analysis of biomolecules - fundamental principles 343
50 Assaying amino acids and proteins 347
51 Assaying lipids 353
52 Assaying carbohydrates 359
53 Assaying nucleic acids and nucleotides 364
54 Protein purification 370
55 Enzyme studies 378
56 Membrane transport processes 390
57 Photosynthesis and respiration 397
Genetics
58 Mendelian genetics 411
59 Bacterial and phage genetics 418
60 Molecular genetics I - fundamental principles 427
61 Molecular genetics II - PCR and related applications 437
62 Molecular genetics III - genetic engineering techniques 443
Analysis and presentation of data
63 Using graphs 453
64 Presenting data in tables 462
65 Hints for solving numerical problems 465
66 Descriptive statistics 475
67 Choosing and using statistical tests 485
Answers to study exercises 497
Index 529.