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Isotope tracers in metabolic research : principles and practice of kinetic analysis /

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Bibliographic Details
Main Author: Wolfe, Robert R.
Other Authors: Chinkes, David L., 1965-
Format: Book
Language:English
Published: Hoboken, N.J. : Wiley-Liss, [2005]
Edition:2nd ed.
Subjects:
Radioactive tracers in biochemistry.
Metabolism > Research > Methodology.
Isotope Labeling > methods.
Metabolism.
Mass Spectrometry > methods.
Online Access:Publisher description
Table of contents
Table of Contents:
  • Basic characteristics of isotopic tracers
  • Calculating specific activity and radiation dosages
  • Calculation of substrate kinetics : single-pool model
  • Calculation of substrate kinetics : multiple-pool model
  • Mass spectrometry : instrumentation
  • Determination of isotopic enrichment
  • Measurement of substrate oxidation
  • Measurement of total energy expenditure using the doubly-labeled water method
  • Mass isotopomer distribution analysis
  • Glucose metabolism
  • Lipid kinetics
  • Whole body protein synthesis and breakdown
  • Measurement of the synthesis of specific proteins
  • Measurement of regional or tissue protein breakdown
  • Arterial-venous balance technique to measure amino acid kinetics
  • Nuclear magnetic resonance.