Real-time trafficking and assembly of ribosomal subunits /

Nuclear pore complexes (NPCs) facilitate the bidirectional transport of a myriad of molecules, ranging in size from an ion to assembled ribosomal subunits, across the two lipid bilayers that constitute the nuclear envelope (NE). Research over the past decade has imparted much insight into how NPCs...

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Bibliographic Details
Main Author: Wang, Jason
Format: Thesis Book
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 2004.
Subjects:
Online Access:Link to OAK Trust copy

MARC

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245 1 0 |a Real-time trafficking and assembly of ribosomal subunits /  |c by Jason Wang. 
264 1 |a [Place of publication not identified] :  |b [publisher not identified] ;  |c 2004. 
300 |a vii, 28 leaves :  |b illustrations ;  |c 28 cm. 
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504 |a Includes bibliographical references (leaves 26-27). 
500 |a Vita. 
500 |a "Major Subjects: Genetics and Biochemistry". 
520 |a Nuclear pore complexes (NPCs) facilitate the bidirectional transport of a myriad of molecules, ranging in size from an ion to assembled ribosomal subunits, across the two lipid bilayers that constitute the nuclear envelope (NE). Research over the past decade has imparted much insight into how NPCs control the selective passage of large macromolecules while maintaining their compartmentalization properties. Schemes for soluble cofactor-mediated active transport have been developed through phenotypic mutational analyses and in vitro experiments. Continuing the effort for a more comprehensive characterization of NPC function, we have developed a method for the in vivo analysis of nucleocytoplasmic transport utilizing fluorescently labeled, recombinant human ribosomal protein L23a (rpL23a-His). Cytoplasmic microinjections into live HeLa cells indicated rapid nuclear import and subsequent export of rpL23a-His. These results are expected to lead to real-time in vivo single-molecule observation of ribosomal subunit trafficking and assembly. 
650 4 |a Majors genetics and biochemistry. 
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