Conjugated energy transfer fluorescent dyes for DNA sequencing /
A set of four fluorescence energy transfer (ET) cassettes wherein the donor and acceptors are connected via a conjugated linker was prepared, and their preliminary spectroscopic properties examined. These cassettes employ fluorescein as the donor and two rosamines and two fluoresceins as the accept...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2003.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=765867481&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A set of four fluorescence energy transfer (ET) cassettes wherein the donor and acceptors are connected via a conjugated linker was prepared, and their preliminary spectroscopic properties examined. These cassettes employ fluorescein as the donor and two rosamines and two fluoresceins as the acceptor dyes. Alkylation of the donor fluorescein carboxylate with 6-bromohexanoic acid provides a handle for attachment to biomolecules. Three of the four ET cassettes have similar intensities when excited at 488 nm. We also studied the viability of using DNA bases as donors in energy transfer dyes. 2'-Deoxyuridine was coupled with acceptor dyes via three consecutively longer conjugated linkers. The corresponding 2'-deoxy- and 2',3'-dideoxynucleoside triphosphates were prepared via aqueous Sonogashira coupling. Extending the conjugated linker resulted in the the nucleoside triphosphates being more efficiently incorporated by DNA Polymerase TaqFS. In addition, the longer linker resulted in increased fluorescence from the acceptor dye when irradiated with UV light. |
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| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xiii, 194 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 76-81). |