rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Phylogenetic analysis of sclerospora graminicola using internal transcribed spaced region-2
  name:
    name part: Viswanathan, Aparna
    name part: 1973-
    role:
      role term: creator
  name:
    name part: Magill, Clint W.
    role:
      role term: thesis advisor.
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 2003
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: remote
    extent: 1 online resource.
  abstract: The internal transcribed spacer region (ITS) from fourteen samples of Sclerospora graminicola was amplified using a nested PCR. These regions of the rDNA were amplified by a combination of conserved primers (ITS 1 and 6 and ITS 3 and 4). These products were cloned into a puc-4 vector and transformed into E.coli competent cells. The inserts from transformants were isolated, then sequenced by the Gene Technologies Lab, Texas A & M University. The sequences produced were and aligned with the ITS2 region of other known Oomycete fungi(Peronospora, Phytophthora and Pythium) using CLUSTALX. Phylogenetic relationship among sequences from Indian and African samples was examined using a consensus bootstrap parsimony tree. All the samples from India, Mali, Nigeria and Niger, with the exception of Niger 4, formed a monophyletic group with the Oomycetes (Peronospora, Phytophthora and Pythium). The three samples Niger 4, BFaso 2 and BFaso 17 from Burkina Faso shared a close relationship with the outgroup Cladosporium herbareum.
  note: by Aparna Viswanathan.
  note: "Major Subject: Plant Pathology."
  note: Title from author supplied metadata.
  note: Electronic resource.
  note: System requirements: Adobe Acrobat Reader.
  note: M.S. Texas A & M University 2003.
  note: Includes bibliographical references.
  note: Text (Thesis).
  subject:
    topic: Major plant pathology
  subject:
    topic: Sclerospora graminicola
  subject:
    topic: phylogenetic analysis
  identifier: http://hdl.handle.net/1969.1/394
  record info:
    record content source: TXA
    record creation date: 040304
    record change date: 20151026051739.0
    record identifier: in00001806611