rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Microvascular function after treatment with an angiogenic factor, TP508, in a porcine model of chronic ischemia
  name:
    name part: Makarski, Lori Ann.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 2003
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    form: remote
    extent: vii, 39 leaves : illustrations ; 28 cm.
  abstract: Therapeutic angiogenesis is a new treatment modality thought to increase blood flow to ischemic areas of myocardium. The objective of this study was to determine if an experimental angiogenic factor, Chysalin® (TP508), enhances the vasodilatory function of microvessels in ischemic myocardium treated with TP508, (treated, TR) when compared to untreated ischemic myocardium (non-treated, NT) and non-ischemic myocardium from control pigs (CON). Adult Yucatan miniature swine were subjected to chronic occlusion of the left circumflex coronary artery (LCX) using an ameroid constrictor. After 28 days, either Pluronic gel (NT, n = 3) or Pluronic gel containing 150 mg of TP508 (TR, n = 8) was injected into the ischemic myocardium. Sixty days after ameroid placement, coronary arterioles (70-100 []m) were isolated from the LCX (ischemic, IS) region and LAD (non-ischemic, NIS) region of the myocardium of TR and NT pigs. Coronary arterioles were isolated from normal myocardium of non-occluded (non-ischemic) pigs and used as controls (n = 5). Microvessels were then tested for functional analysis using the vasodilators adenosine, serotonin, pinacidil, and nitroprusside. Coronary arterioles (100-300 []m in diameter) were isolated for nitric oxide (NO) analysis and eNOS gene expression using an RT/PCR technique. In our study, smooth muscle relaxation of coronary microvessels was significantly decreased in the IS region of NT animals compared to controls when assayed with adenosine and serotonin (p < 0.05), but no difference existed between CON and TR groups (p>0.05). With nitroprusside and pinacidil, no differences in dilation were observed between the three groups, suggesting a lack of endothelial NO-mediated dilation in the IS/NT group. Treated microvessels isolated for NO analysis showed a significant increase in NO production as compared to the IS/NT group (p < 0.05). Endothelial nitric oxide synthase (eNOS) mRNA expression was not significantly different between groups, suggesti…
  note: by Lori Ann Makarski.
  note: "Major subject: Veterinary Medical Sciences".
  note: In title numerals are used.
  note: Vita.
  note: Also available online.
  note: Issued also on microfiche from Lange Micrographics.
  note: M.S. Texas A&M University 2003
  note: Includes bibliographical references (leaves 35-38).
  subject:
    topic: Major veterinary medical sciences
  identifier: https://hdl.handle.net/1969.1/ETD-TAMU-2003-THESIS-M362
  related item:
    location:
      url: https://hdl.handle.net/1969.1/ETD-TAMU-2003-THESIS-M362
  record info:
    record content source: TXA
    record creation date: 031119
    record change date: 20220103114316.0
    record identifier: in00001787890