Molecular studies on fiber initiation genes in cotton (Gossypium hirsutum L.) /
Improving fiber yield and quality has the highest priority in cotton production. However, the molecular basis for the initiation of fiber cells is poorly understood. Fiber cells are developed from the epidermal surface of fertilized ovule or maternal tissue. Conventional approaches are focused on s...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2002.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=765073191&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Improving fiber yield and quality has the highest priority in cotton production. However, the molecular basis for the initiation of fiber cells is poorly understood. Fiber cells are developed from the epidermal surface of fertilized ovule or maternal tissue. Conventional approaches are focused on studying genes involved in secondary wall biosyntheses in the late stages of fiber development. The biological process during fiber initiation is unclear, due to lack of proper techniques for study. We have developed an AFLP-cDNA display method to analyze transcription profiles in cotton. Using the AFLP-cDNA method, we performed a genome-wide survey of gene expression profiles in fiber initiation stages. By comparing cDNA profiles in leaves, petals, ovules, and young fiber tissues in cotton, we have identified dozens of cDNA fragments present in mature ovules and young fiber cells but absent in leaves and petals. The expression patterns of the candidate genes were verified in fiber and non-fiber tissues using RT-PCR and real time PCR. The genes and expression patterns are useful in understanding the genetic basis of fiber initiation and improving cotton production by manipulating expression of the genes essential for fiber cell development. |
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| Item Description: | Vita. "Major Subject: Plant Breeding". |
| Physical Description: | ix, 80 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 67-79). |