AFLP-based study of genetic difference and mega-base DNA isolation for BAC library construction from greenbug Schizaphis graminum (Rondani) (Homoptera:Aphididae) /
This research focuses on the study of genetic differences among eight greenbug biotypes revealed by AFLP analyses and isolation of mega-base DNA for BAC library construction. Genetic relationship among greenbug biotypes is an old, but interesting topic. Understanding of genetic differences could hel...
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| Format: | Thesis eBook |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2002.
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| Online Access: | Link to OAKTrust copy |
| Summary: | This research focuses on the study of genetic differences among eight greenbug biotypes revealed by AFLP analyses and isolation of mega-base DNA for BAC library construction. Genetic relationship among greenbug biotypes is an old, but interesting topic. Understanding of genetic differences could help in elucidating roles of greenbug-host interaction in plant resistance and assist in development of new resistant cultivars. In addition, constructing a BAC library for greenbug will provide a powerful tool for molecular dissection of counter-defense mechanisms. Genetic relationship analyses based on 3341 AFLP markers showed that biotypes damaging sorghum cultivars and hybrids (biotypes C, E, I and K) shared higher genetic similarity as compared to non-sorghum biotypes. This result may provide a way of using AFLP fingerprinting for prediction of potential sorghum greenbugs and may lead to more effective and longer lasting plant resistance. An efficient protocol suitable for mega-base DNA isolation from greenbug (Schizaphis graminum) (Rondani) was developed. The resulting high quality mega-base DNA from greenbug was used to construct a BAC library. The average insert size of the library was estimated to be 96kb, with an average frequency of false positive clones of 19%. |
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| Item Description: | "Major subject: Molecular and Environmental Plant Sciences". Vita. |
| Physical Description: | x, 62 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references (leaves 55-61). |