rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Proteomic analysis of E. coli using 2D HPLC and MALDI-TOF mass spectrometry
  name:
    name part: Campbell, Christopher S.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 2002
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: viii, 13 leaves : illustrations ; 28 cm.
  abstract: In this post-genomic era, researchers are striving to find new ways to use the enormous amounts of data that have been collected. One obvious way is with proteomics, the large-scale identification of expressed proteins. We have developed a novel method for identifying proteins using two dimensions of non-denaturing high performance liquid chromatography (HPLC) and matrix assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry. The first dimension of separation uses an anion exchange column and each of those fractions is run through the second dimension, a hydrophobic interaction column. The proteins were then dialyzed, denatured, and digested with trypsin before being subjected to mass spectrometry. Identifications were made based on the peptide masses. Using this method we have made 2012 protein identifications, 310 of which are unique. These numbers are comparable to other forms of proteomics such as 2-D gels.
  note: by Christopher S. Campbell.
  note: Vita.
  note: In title numerals are used.
  note: "Major Subject: Life Sciences I".
  note: Fellows Thesis Texas A&M University 2002.
  note: Includes bibliographical references (leaves 12-13).
  subject:
    topic: Major life sciences I
  identifier: http://hdl.handle.net/1969.1/ETD-TAMU-2002-Fellows-Thesis-C35
  related item:
    location:
      url: http://hdl.handle.net/1969.1/ETD-TAMU-2002-Fellows-Thesis-C35
  record info:
    record content source: TXA
    record creation date: 030605
    record change date: 20151025071524.0
    record identifier: in00001755916