Molecular and cytological analysis of a novel leaf rust resistance gene in wheat /
A novel wheat leaf rust resistance gene from Aegilops cylindricum accession TTCC295 was investigated, using both cytogenetic and molecular tools. Previous work had introgressed this gene into adapted wheat germplasm, which was crossed to 'Chinese Spring' stocks monosomic for all 7 of the...
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
2002.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | A novel wheat leaf rust resistance gene from Aegilops cylindricum accession TTCC295 was investigated, using both cytogenetic and molecular tools. Previous work had introgressed this gene into adapted wheat germplasm, which was crossed to 'Chinese Spring' stocks monosomic for all 7 of the D-genome chromosomes. These populations were scored for reaction to leaf rust, and the inheritance of the resistance proved to be dominant and monogenic. The leaf rust resistance gene was determined by Chi-square analysis to be on chromosome 2D. Subsequent work using a series of microsatellite markers associated with this chromosome confirmed their location on 2D, and further established that the gene was on the extreme distal portion of the chromosome. A linkage map was constructed of the region near the gene, but, since the nearest available marker was 10.7 cM proximal to the resistance gene, the markers are probably too distant to be of use in an applied breeding program using marker assisted selection. |
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| Item Description: | "Major subject: Plant Breeding". Vita. |
| Physical Description: | vii, 42 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references (leaves 32-36). |