Detection of Rhodococcus equi by polymerase chain reaction using species-specific, non-proprietary primers /
Species-specific primers for the polymerase chain reaction (PCR) for the detection of Rhodococcus equi were developed. These primers were based on unique DNA fragments produced from R. equi reference strains and field isolates. Following random amplification of polymorphic DNA (RAPD) from R. equi...
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| Format: | Thesis eBook |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2002.
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| Online Access: | Link to OAKTrust copy |
| Summary: | Species-specific primers for the polymerase chain reaction (PCR) for the detection of Rhodococcus equi were developed. These primers were based on unique DNA fragments produced from R. equi reference strains and field isolates. Following random amplification of polymorphic DNA (RAPD) from R. equi and R. rhodochrous with a set of 40 arbitrary 10-bp primers, a pair of species-specific primers was designed to detect a unique 700-bp fragment of R. equi chromosomal DNA. This PCR product was limited to amplification of DNA from R. equi and was not detectable in DNA extracted from other Rhodococcus species or from a panel of additional gram-positive and gram-negative bacteria. |
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| Item Description: | "Major subject: Veterinary Microbiology". Vita. |
| Physical Description: | ix, 39 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references (leaves 22-28). |