Identification and elucidation of chemical modifications of DNA oligonucleotides using high resolution time-of-flight mass spectrometry /
Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) coupled to time-of-flight mass spectrometry (TOFMS) are used to characterize native and chemically modified DNA oligonucleotides. First, improvements on sample preparations are shown. Then, the utility of these s...
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
2002.
|
| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=764789761&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) coupled to time-of-flight mass spectrometry (TOFMS) are used to characterize native and chemically modified DNA oligonucleotides. First, improvements on sample preparations are shown. Then, the utility of these sample preparations is shown for oligonucleotide mixture analysis. Accurate mass measurement of DNA oligonucleotides is evaluated using MALDI and ESI TOFMS with two commercial instruments. Using accurate mass measurents and different fragmentation mechanisms, chemical modifications of DNA are examined. Cytosine reduction by 2,5-dihydroxybenzoic acid matrix is studied using MALDI TOFMS and in-source decay (ISD) techniques. Dirhodium and platinum adducts are examined with MALDI and ESI TOFMS; in addition, ISD, collision-induced dissociation tandem mass spectrometry (CID MS-MS), and enzymatic digestion are used to further characterize these metal adducts. |
|---|---|
| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xvii, 214 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 192-213). |