Lipoproteomics : multi-dimensional analysis of human serum apolipoproteins /
A method for the two-dimensional (2D) gel analysis of apolipoproteins was developed using commercial apolipoprotein standards and applied to the analysis of lipoprotein fractions collected from an ultracentrifugation (UC) spin. A term lipoproteomics was introduced as the characterization of the pro...
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
2002.
|
| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=764785941&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A method for the two-dimensional (2D) gel analysis of apolipoproteins was developed using commercial apolipoprotein standards and applied to the analysis of lipoprotein fractions collected from an ultracentrifugation (UC) spin. A term lipoproteomics was introduced as the characterization of the proteins associated with the lipoprotein fractions. Three analytical techniques including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), immobilized pH gradient isoelectric focusing (IPG-IEF), and two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-D SDS-PAGE) comprised the core of the lipoproteomics protocols. These methods were used to analyze the very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL), and bottom fractions from the UC spin. High sensitivity and baseline resolution was achieved with the MALDI-TOF-MS analysis, and novel isoforms of the SAA₄ protein were detected in the HDL fraction. The IPG-IEF separation was optimized for sharp focused bands and high resolution of the protein spots in the second dimension. Low abundant proteins were detected with the 2-D gel analysis of the lipoprotein fractions with silver staining. The lipoproteomic approach was applied to a clinical population in search for markers for the determination of success of percutaneous transluminal angioplasty (PTCA) in an individual. |
|---|---|
| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xvi, 203 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 148-182). |