rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: isolation and characterization of a peptide synthetase gene from Trichoderma virens
  name:
    name part: Wiest, Aric.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 2002
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    form: remote
    extent: x, 65 leaves : illustrations ; 28 cm.
  abstract: The filamentous fungus Trichoderma has a long history of biological control, protecting numerous plant species against a broad spectrum of soilborne pathogens. Additionally, many species are significant sources of industrial enzymes. Trichoderma isolates have been shown to produce a number of antibiotic compounds, including peptaibols, which are deleterious to both fungi and bacteria. Peptaibols are known for their high α-aminoisobutyric acid content and their production as a mixture of isoforms ranging from 7-20 amino acids in length. The antibiotic activity they exhibit is probably due to an ability to form pores in lipid membranes. These pores conduct ions, leading to loss of osmotic balance and cell death. Because of the potential importance of peptaibols in the biological control of plant diseases, we sought to clone the gene(s) responsible for their synthesis. With their unusual amino acid content, we expected peptaibols to be the product of nonribosomal peptide synthetases (NRPSs). NRPSs have a modular structure in which each module is a semiautonomous unit that recognizes, activates, and incorporates a single residue into the final peptide. We have identified novel peptaibols from Trichoderma virens and cloned the NRPS gene, tex1, responsible for their biosynthesis. Disruption of tex1 in T. virens eliminates peptaibol production, as determined by HPLC analysis. This gene is the first complete peptide synthetase gene cloned from the genus Trichoderma. The 62.8 kb continuous open reading frame represents not only the first peptaibol synthetase gene cloned, but also the largest known open reading frame of any gene in any organism. Tex1 is expressed under all examined conditions, including ungerminated conidia. Since modular order is co-linear with that of the product, we were able to assign substrate amino acid(s) to each module. Comparison of protein sequences between modules is consistent with the module/substrate assignments. Here we report the first genetic…
  note: by Aric Wiest.
  note: "Major subject: Plant Pathology".
  note: Vita.
  note: Also available online.
  note: Issued also on microfiche from Lange Micrographics.
  note: M.S. Texas A&M University 2002
  note: Includes bibliographical references (leaves 47-53).
  subject:
    topic: Major plant pathology
  identifier: https://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W343
  related item:
    location:
      url: https://hdl.handle.net/1969.1/ETD-TAMU-2002-THESIS-W343
  record info:
    record content source: TXA
    record creation date: 030204
    record change date: 20220103114316.0
    record identifier: in00001724563