| Tag |
First Indicator |
Second Indicator |
Subfields |
| LEADER |
00000ctm a2200000Ka 4500 |
| 001 |
in00001661890 |
| 005 |
20220103114501.0 |
| 007 |
cr unu a |
| 008 |
020402s2001 xx a b 000 0 eng d |
| 035 |
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|a (OCoLC)ocm49502298
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| 040 |
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|a TXA
|c TXA
|d UtOrBLW
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| 049 |
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|a TXAM
|a TXAR
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| 099 |
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|a 2001
|a Thesis
|a B353
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| 100 |
1 |
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|a Barenboim, Maxim,
|d 1967-
|
| 245 |
1 |
0 |
|a Characterization of the dual start motif of a class II holin gene /
|c by Maxim Barenboim.
|
| 264 |
|
1 |
|a [Place of publication not identified] :
|b [publisher not identified] ;
|c 2001.
|
| 300 |
|
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|a ix, 70 leaves :
|b illustrations ;
|c 28 cm.
|
| 336 |
|
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|a text
|b txt
|2 rdacontent
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| 337 |
|
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|a computer
|b c
|2 rdamedia
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| 338 |
|
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|a online resource
|b cr
|2 rdacarrier
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| 500 |
|
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|a "Major subject: Biology".
|
| 500 |
|
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|a Vita.
|
| 502 |
|
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|b M.S.
|c Texas A&M University
|d 2001.
|
| 504 |
|
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|a Includes bibliographical references (leaves 63-69).
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| 520 |
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|a Holins are small membrane proteins, which, at a genetically programmed time in a bacteriophage infective cycle, allow bacteriolytic enzymes, or endolysins, to escape to the periplasm and attack the cell wall. Most holins fall into two sequence classes, I and II, based on the number of potential transmembrane domains (3 for class I and 2 for class II). The prototype class I holin gene, S[], has a dual start motif and encodes not only the effector holin S[]105 but also an inhibitor S[]107 with a Met-Lys...extension at the N-terminus. The prototype class II holin gene of phage 21, S²¹, begins with the motif Met-Lys-Ser-Met...; a potential RNA secondary structure overlaps the Shine-Dalgarno sequence. Here, I demonstrate that (1) two protein products are elaborated from S²¹, S²¹71, and S²¹68; (2) the shorter product is required for lysis; (3) the longer product, S²¹71, inhibits S²¹ function; and (4) the Lys₂ residue is important for the inhibitor function. Moreover, it is shown that the RNA stem-loop structure is involved in the down-regulation of S²¹71 synthesis and that in S²¹ different segments of the single consensus Shine-Dalgarno sequence serve the two translational starts. It is concluded that the dual start motifs of class II holin genes are functionally homologous to those of class I holin genes but have a fundamentally different molecular basis.
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| 530 |
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|a Also available online.
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| 530 |
|
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|a Issued also on microfiche from Lange Micrographics.
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| 650 |
|
4 |
|a Major biology.
|
| 856 |
4 |
1 |
|u https://hdl.handle.net/1969.1/ETD-TAMU-2001-THESIS-B353
|z Link to OAKTrust copy
|t 0
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| 948 |
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|a cataloged
|b h
|c 2002/4/2
|d c
|e eneff
|f 12:22:15 pm
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| 994 |
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|a E0
|b TXA
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| 999 |
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|a MARS
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| 999 |
f |
f |
|s d77432b7-69e0-3c03-a2ce-bce61d893a0b
|i 07ad343a-f1ab-3c1b-981d-fa46756e5983
|t 0
|
| 952 |
f |
f |
|p noncirc
|a Texas A&M University
|b College Station
|c Cushing Memorial Library & Archives
|s cush tdrm
|d Cushing: Theses & Dissertations Microforms (Does not check out)
|t 0
|e 2001 Thesis B353
|h Other scheme
|i computer -- online resource
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| 952 |
f |
f |
|a Texas A&M University
|b College Station
|c Electronic Resources
|s www_evans
|d Available Online
|t 0
|e 2001 Thesis B353
|h Other scheme
|
| 998 |
f |
f |
|a 2001 Thesis B353
|t 0
|l Cushing: Theses & Dissertations Microforms (Does not check out)
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| 998 |
f |
f |
|a 2001 Thesis B353
|t 0
|l Available Online
|