Examination of interactions between vitamin C and vitamin E and effects on immune responses and disease resistance of hybrid striped bass, Morone chrysops x Morone saxatilis /
A series of experiments was conducted to investigate the presence of an in vivo interaction between dietary vitamin C and vitamin E in hybrid striped bass. Additional experiments were conducted to examine the effects of vitamin C and vitamin E on immune function in vitro and in vivo including diseas...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=727847521&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A series of experiments was conducted to investigate the presence of an in vivo interaction between dietary vitamin C and vitamin E in hybrid striped bass. Additional experiments were conducted to examine the effects of vitamin C and vitamin E on immune function in vitro and in vivo including disease resistance following experimental infection with Streptococcus iniae. Juvenile hybrids were fed one of nine semipurified diets supplemented with either 0, 25, or 2500 mg vitamin C kg⁻¹ and 0, 30, or 300 mg vitamin E kg⁻¹ for 10 weeks. Dietary vitamin C at inclusion levels of 25 and 2500 mg kg⁻¹ improved feed efficiency and protected fish fed vitamin E-deficient diets from growth depression and mortality. Vitamin E at inclusion levels of 30 and 300 mg kg⁻¹ prevented mortality in fish fed vitamin C-deficient diets and 300 mg vitamin E kg⁻¹ was necessary to prevent growth depression of fish fed vitamin C-deficient diets but was unable to improve feed efficiency. These data provide support for an in vivo interaction between vitamin C and vitamin E in hybrid striped bass. Nonspecific immune functions were examined to characterize the immune responses of hybrid striped bass fed either 0, 25, or 2500 mg vitamin C kg⁻¹ and 0, 30, or 300 mg vitamin E kg⁻¹ for 10 weeks. Respiratory burst response also was investigated in vitro to characterize the mechanism by which vitamin C and vitamin E alter phagocyte function. Megadose levels of vitamin C and/or vitamin E were further examined to determine the practical application of these nutrients to improve survival following infection with Streptococcus iniae. Although stimulatory effects of vitamin C and E on in vitro intracellular superoxide production were observed, no significant corresponding improvements in bacterial killing ability or fish survival following experimental infection were realized. In summary, results of this research have established both in vitro and in vivo interactions between vitamin C and vitamin E in hybrid striped bass. However, elevated dietary concentrations of vitamins C and E had limited effects on immune function and disease resistance of hybrid striped bass. |
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| Item Description: | Vita. "Major Subject: Nutrition". |
| Physical Description: | xii, 99 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 86-98). |