Construction and analysis of two ordered BAC arrays spanning the bovine major histocompatability complex (BoLA) /
The major histocompatibility complexes (MHCs) of mice (H-2) and humans (HLA) are among the most completely characterized regions of the mammalian genomes. Genes of the MHC have been associated with the increased susceptibility to a wide range of diseases, including anklyosing spondylitis, multiple...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2001.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=728907631&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The major histocompatibility complexes (MHCs) of mice (H-2) and humans (HLA) are among the most completely characterized regions of the mammalian genomes. Genes of the MHC have been associated with the increased susceptibility to a wide range of diseases, including anklyosing spondylitis, multiple sclerosis, psoriasis, and insulin-dependent diabetes mellitus. Genes of the MHC also function in spermatogenesis, early embryonic development, immune responsiveness, resistance to viral infection, and the predisposition to the formation of tumors and metastasis. Significant rearrangements have occurred over evolutionary time between H-2 and HLA to alter gene numbers and arrangements, but the genetic content of H-2 and HLA remains essentially intact and accommodated within about 4 Mb of DNA. In contrast, genes of the bovine MHC (BoLA) occur in two clusters separated by a genetic distance of about 20 cM. Evidence suggests that a single large inversion is responsible for the disruption of BoLA (Band et al. 1998). The telomeric breakpoint of the inversion occurred within the class II region near the contemporary DQ locus giving rise to the class IIa subregion at 23q21 and the class IIb subregion at 23q12. The position of the centromeric breakpoint is just telomeric of the bovine homologue of the human EST AA298919 (Newkirk et al. submitted). To better characterize the organization of BoLA, we have isolated 39 bovine bacterial artificial chromosomes (BAC) clones that contain genes homologous to HLA class I, II, and III loci. Two ordered arrays have been assembled that span BoLA, one across the class IIb subregion and one across the class IIa, III, and I region. This study will report on the construction and analysis of the genetic organization of both BoLA arrays |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xv, 275 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 184-199). |