Canine digestive lipases /
Canine gastric lipase (cGL) was purified from gastric mucosa of a dog and partially characterized. The molecular mass of cGL was 49 kDa and the isoelectric point range 6.8 to 6.9. N-terminal amino acid sequence for the first 25 amino acids was identical to that reported previously. Evidence for the...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=728408751&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Canine gastric lipase (cGL) was purified from gastric mucosa of a dog and partially characterized. The molecular mass of cGL was 49 kDa and the isoelectric point range 6.8 to 6.9. N-terminal amino acid sequence for the first 25 amino acids was identical to that reported previously. Evidence for the formation of multimers of cGL in vitro was identified. Canine pancreatic lipase (cPL) was purified from dog pancreas and also partially characterized. Canine pancreatic lipase had a molecular mass of 50.7 kDa, a specific absorbance of 1.11 at a wavelength of 280 nm for a 1 mg/ml solution, a specific activity of 6,288 U/mg, an isoelectric point range of 6.0 to 6.2, and a N-terminal amino acid sequence that shows great homology to classical pancreatic lipase of other species. Antisera directed against cGL and cPL were raised in New Zealand White rabbits, and purified by affinity chromatography. Immunolocalization in various tissue specimens from a healthy dog revealed staining for cGL only in mucous neck and mucous pit cells of the gastric glands. Similarly, immunolocalization for cPL showed staining in pancreatic acinar cells only. An enzyme-linked immunosorbent assay (ELISA) for measurement of canine gastric lipase immunoreactivity (cGLI) in serum was developed and validated. Also, a radioimmunoassay for canine pancreatic lipase immunoreactivity (cPLI-RIA) was developed and validated. Finally, an ELISA for measurement of serum cPLI (cPLI-ELISA) concentration was developed and validated. Median serum cGLI concentration was not significantly different between clinically healthy dogs and dogs with gastritis and only 3 of 25 dogs with gastritis had serum cGLI concentrations above the upper limit of the reference range. Thus serum cGLI concentration was not clinically useful for the diagnosis of gastritis in the dog. Serum cPLI concentration proved to be a specific marker for exocrine pancreatic function and was consistently decreased in dogs with exocrine pancreatic insufficiency, when measured by ELISA. Initial data suggest that serum cPLI concentration is useful for the diagnosis of canine pancreatitis. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". |
| Physical Description: | xiv, 251 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 204-250). |