Genetic reprogramming in bovine embryos produced by somatic cell nuclear transfer /
The production of live offspring following NT depends on reprogramming of gene expression by the recipient oocyte from that found in the donor cell to one compatible with embryonic and fetal growth. This study analyzed mRNA expression in NT embryos and fetuses to evaluate genetic reprogramming. All...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=728331921&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The production of live offspring following NT depends on reprogramming of gene expression by the recipient oocyte from that found in the donor cell to one compatible with embryonic and fetal growth. This study analyzed mRNA expression in NT embryos and fetuses to evaluate genetic reprogramming. All three lactate dehydrogenase transcripts were detected in 8-cell IVF and NT embryos. In blastocyst stage NT and IVF embryos LDH-A and LDH-B were detected but LDH-C was not detected. LDH-C was detected in both NT and in vivo fetal gonad. Phosphofructokinase transcripts were not detected in 8-cell stage embryos but were detected at the blastocyst stage from IVF and NT produced embryos. Citrate synthase mRNA was detected throughout preattachment IVF development and was detected in 8-cell and blastocyst stage NT embryos. IGF-II mRNA expression was greater in NT blastocysts produced using cloned fetal fibroblasts as NT donor cells than from in vivo and IVF controls and from adult cell NT blastocysts. IGF-IIR mRNA expression at the blastocyst stage was not different. IGF-II mRNA expression was lower in placenta from an NT fetus produced from cloned fetal fibroblast cells than from an in vivo control. IGF-II mRNA expression was not different between the NT and control fetal liver however IGF-IIR mRNA was lower in the NT fetus compared to control. Northern blot analysis revealed 6 different IGF-II mRNA species at 5.2, 4.0, 2.8, 2.3, 1.7, and 1.3 kb for both NT and control fetal livers. Fetal fibroblast cells expressed the 5.2 and 4.0 IGF-II bands but were not detected in adult cells. Two major IGF-II bands were detected in cotyledon at 5.2 and 2.8 kb and caruncular tissue at 5.2 and 4.0 kb. This study largely supports reprogramming of the three metabolic enzymes and the IGF-II and IGF-IIR mRNA. The exception being the elevated IGF-II mRNA levels detected in blastocysts and the lower IGF-IIR mRNA seen in the fetal livers from cloned fetal fibroblast NT. IGF-II mRNA expression was significantly greater in the fetal fibroblast cells compared to the adult cells and this disparity could explain the differences seen at later stages of NT development. |
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| Item Description: | Vita. "Major Subject: Veterinary Physiology". |
| Physical Description: | xii, 104 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 91-102). |