Charge density profiling of human LDL particles by capillary zone electrophoresis /
Capillary zone electrophoresis (CZE) has been utilized to profile the LDL particles in human blood serum in this study. During the method development phase, three buffers (sodium borate, phosphate buffered saline and sodium phosphate) with different pH and ionic strength were investigated. Influence...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=727726311&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Capillary zone electrophoresis (CZE) has been utilized to profile the LDL particles in human blood serum in this study. During the method development phase, three buffers (sodium borate, phosphate buffered saline and sodium phosphate) with different pH and ionic strength were investigated. Influences of buffer compositions, pH and ionic strength on the LDL profile have been thoroughly studied. Influence of stained and unstained serum for the preparation of LDL samples was also investigated. In the final step, 5 mM sodium phosphate buffer, pH 7.40 was chosen as the most suitable CE buffer and an extensive ultrafiltration (UF) procedure was applied to purify the LDL sample. Two LDL particle species, LDL with lower mobility and LDL ̄with higher mobility were observed. The electropherograms were highly reproducible with good precision of effective mobilities, corrected peak area (CPA) and CPA ratio of LDL/̄LDL. LDL particles shown on the electropherogram were also characterized by several experiments. The applications of Sigma HDL cholesterol reagent and CE on-line 2-propanol precipitation indicated that the two particle species shown in the electropherogram belong to LDL. The LDL particles were found to associate with the bouyant LDL fraction and the LDL ̄particles associate with the dense LDL fraction. The electropherogram patterns were significantly different between the apparently healthy subjects and patients with carotid atherosclerotic disease. The LDL ̄region is complex and with a wide distribution for the patients. The CPA ratio of LDL/̄LDL is also significantly low for the patients. The method developed in this study is the first report for the profiling of LDL isoforms by capillary zone electrophoresis. This finding provides a new analytical method for the resolution of sub-species of LDL that may be markers for the existence of plaque in the arterial wall. |
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| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xii, 90 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 76-81). |