Differential activation of bovine T lymphocyte subpopulations in response to mitogen and superantigen stimulation /
This study examined the response of bovine CD4⁺, CD8⁺, and TCR γ[] T lymphocytes to activation with the mitogens concanavalin A (Con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM), and the superantigens Staphylococcus enterotoxin A (SEA) and SEB. Several molecules (CD25, CD71, ACT1, PCNA,...
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| Format: | Thesis eBook |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | This study examined the response of bovine CD4⁺, CD8⁺, and TCR γ[] T lymphocytes to activation with the mitogens concanavalin A (Con A), phytohemagglutinin (PHA), and pokeweed mitogen (PWM), and the superantigens Staphylococcus enterotoxin A (SEA) and SEB. Several molecules (CD25, CD71, ACT1, PCNA, cyclin D, cdk4, and cdk6) were tested for suitability as activation markers. The marker of activation chosen was CD25, the interleukin-2 receptor α chain. Cells were analyzed by flow cytometry, and the mean fluorescence intensity ratio was determined. A dose response was found to each activating agent in all 3 T cell subsets. A relationship between time in culture with the activating agent and CD25 expression was found to exist in CD4⁺ and TCR []γ cells, but not CD8⁺ cells. This study documents, for the first time, mitogen- and superantigen-induced activation of bovine T cell subsets at early time points. |
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| Item Description: | "Major subject: Veterinary Microbiology". Vita. |
| Physical Description: | ix, 79 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references (leaves 71-78). |