Cloning and characterization of the Aspergillus nidulans spdA gene /
Using a PCR product amplified from Aspergillus nidulans DNA as a probe, the A. nidulans spdA gene, coding for a putative spermidine synthase, was isolated. Alignment of its deduced amino acid sequence with those of other spermidine synthases showed a high degree of identity. Northern blot analysis s...
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| Format: | Thesis eBook |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Subjects: | |
| Online Access: | Link to OAKTrust copy |
| Summary: | Using a PCR product amplified from Aspergillus nidulans DNA as a probe, the A. nidulans spdA gene, coding for a putative spermidine synthase, was isolated. Alignment of its deduced amino acid sequence with those of other spermidine synthases showed a high degree of identity. Northern blot analysis showed that the spdA mRNA accumulates at constant levels. This suggests that spermidine synthase transcript levels are not differentially regulated during development. To investigate the function of spermidine on differentiation and sterigmatocystin (ST) production of A. nidulans, we constructed a A. nidulans spdA null mutant by replacing a portion of the coding region with the argB gene. The spdA null mutant strain has an absolute requirement for spermidine for vegetative growth, sporulation, and ST production. Overexpression of flbA in the spdA null mutant strain activated conidiation and ST production. This result supports the hypothesis that polyamine effects on sporulation and ST production is mediated in part through the FlbA/FadA signaling pathway. |
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| Item Description: | "Major subject: Plant Pathology". Vita. |
| Physical Description: | ix, 55 leaves : illustrations ; 28 cm. Also available online. Issued also on microfiche from Lange Micrographics. |
| Bibliography: | Includes bibliographical references (leaves 48-54). |