Capillary electrophoresis of human serum apolipoproteins /
The effective mobilities of commercial apolipoproteins were determined using capillary electrophoresis (CE) and a high pH buffer. Three different lipoprotein delipidation methods were used in conjunction with CE to profile the apolipoproteins in VLDL, LDL, and HDL fractions from human serum and det...
| Main Author: | |
|---|---|
| Format: | Thesis Book |
| Language: | English |
| Published: |
[Place of publication not identified] :
[publisher not identified] ;
2000.
|
| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=727720971&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | The effective mobilities of commercial apolipoproteins were determined using capillary electrophoresis (CE) and a high pH buffer. Three different lipoprotein delipidation methods were used in conjunction with CE to profile the apolipoproteins in VLDL, LDL, and HDL fractions from human serum and determine feasibility for apo quantitation by CE. The delipidation methods included methanol-diethyl ether liquid-liquid extraction, sodium dodecyl sulfate (SDS) delipidation, and a new method of delipidation involving the use of a reversed phase C₁₈ solid phase extraction (SPE) cartridge. The SDS method of delipidation was the only method suitable for the analysis of apo B-100, the major protein in the VLDL and LDL fractions. However, the SDS method provided incomplete delipidation of the HDL fraction. The two other delipidation methods provided complete delipidation of the HDL fraction; however, the C₁₈-SPE method of delipidation produces a higher and more reproducible protein yield than the conventional liquid-liquid methanol-ether delipidation technique. Furthermore, the C₁₈-SPE method implements a fast, sequential, desalting and delipidation of the lipoproteins for subsequent CE and mass spectrometric analysis providing high quality electropherorograms and mass spectra. In addition, the C₁₈-SPE method was applied toward the quantitation of the major proteins of the HDL fraction and the profiling of healthy individuals and individuals with known coronary heart disease. |
|---|---|
| Item Description: | Vita. "Major Subject: Chemistry". |
| Physical Description: | xiii, 222 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 196-218). |