http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Discovery and characterization of novel capillary morphogenesis genes and analysis of differential gene expression during endothelial cell tube formation in three-dimensional collagen matrices
The importance of blood vessel formation and its implications in development and pathogenic states have caused a surge in research aimed at creating a better understanding of this intricate process. Some of the key molecular interactions that are involved have been well described. The technologies and assays used in combinatorial fashions to study the angiogenic process have yielded results that allow perspective to be gained of the interplay between interactions of soluble and extracellular matrix molecules and their receptors and the resulting downstream signaling events. Using a system of capillary morphogenesis developed in our laboratory, we have analyzed differential gene regulation during endothelial tube formation by three methods, 1) DNA microarray technology, 2) differential display analysis, and 3) morphogenic endothelial cDNA library screening. These investigations were done over a time course including 0 hours, 8 hours, 24 hours, and 48 hours of progression of capillary-like tube formation by endothelial cells. In situ hybridization has implicated many of these genes during angiogenesis in vivo. From the DNA microarray analysis we identified many known genes - of which some had previously been implicated during angiogenic and capillary morphogenic events and some had not - to be dramatically regulated as endothelial morphogenesis progressed. Many gene groups displayed various up and down regulation during the time course analysis, possibly indicating roles in establishing a balance between positive and negative regulation. Other groups of genes showed particular propensities toward promoting or inhibiting certain cellular events as morphogenesis progressed. In addition to the known genes described above, a large number of novel genes were isolated, which we have termed capillary morphogenesis genes (CMGs), using differential display and morphogenic endothelial cDNA library screening approaches. Among the novel genes discovered, three have had the full length message identified and their functions are being elucidated at present. The structures were analyzed and initial characterizations were made for two of the novel genes, CMG1 and CMG2, discovered during capillary morphogenesis, which are described in the following report.
Issued also on microfiche from University Microfilm Inc.
Includes bibliographical references (leaves 114-126).