rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    non sort: The
    title: low-copy component of the Pinus taeda genome
  name:
    name part: Elsik, Christine Golemboski.
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 2000
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    extent: xii, 92 leaves : illustrations ; 28 cm.
  abstract: I have taken an alternative approach to characterize a large plant genome: sequence-based analysis of noncoding DNA. First, I have provided insight into the evolutionary processes occurring in noncoding DNA by experimentally testing the models of DNA turnover and fossil repeat contribution to low-copy DNA. Second, I have improved the basic understanding of the pine genome using a novel low-copy enrichment method. The pine genome's large size (> 20,000 Mb/1C) and conserved karyotype make it a model for basic genome organization in higher plants. Its low-copy kinetic component is in great excess to the DNA amount required for gene expression. The original Pinus strobus C₀T curve was reanalyzed using a new P. strobus genome size estimate, indicating that previous estimates of the excess low-copy component were upwardly biased. P. taeda low-copy sequences were sampled, revealing that diverged retroelements contribute to the excess low-copy DNA. Fossil repeats in the low-copy component of the pine genome were identified using a sequence-based approach. The pine genome's large (> 75%) repetitive DNA fraction impedes molecular approaches. Thus, a low-copy enrichment method was developed to facilitate efforts to characterize low-copy DNA and develop markers. The low-copy enrichment method, based on reassociation kinetics and hybrid capture, was shown to enrich for P. taeda low-copy sequences. The high proportion of repetitive DNA in the pine genome has hindered microsatellite marker development. Low-copy enrichment was tested as a method to improve marker recovery. Recovery of useful markers per sequence tested was higher from microsatellite-selected libraries constructed using P. taeda low-copy enriched DNA fragments than from total-genome microsatellite-selected libraries. A survey of the entire P. taeda range showed that the low-copy enrichment did not reduce allelism or polymorphic information content. The low-copy enrichment method provided a means to test nonrandom di…
  note: by Christine Golemboski Elsik.
  note: Vita.
  note: "Major Subject: Genetics".
  note: Issued also on microfiche from University Microfilm Inc.
  note: Ph. D. Texas A&M University 2000.
  note: Includes bibliographical references (leaves 81-89).
  subject:
    topic: Major genetics
  identifier: 99-68909 UMI
  identifier: http://proxy.library.tamu.edu/login
  related item:
    location:
      url: http://proxy.library.tamu.edu/login
  record info:
    record content source: TXA
    record creation date: 010209
    record change date: 20190325101044.0
    record identifier: in00001583995