In vitro studies on broad spectrum antiviral agents for the control of bovine rotavirus and coronavirus infections /
Rotaviruses are the leading cause and coronaviruses are major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling in...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1999.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=731686641&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | Rotaviruses are the leading cause and coronaviruses are major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling infection, these studies are an investigation of broad-spectrum antiviral agents that do not discriminate against various viruses. One part of this study involves testing a variety of adsorbent agents including charcoal, clay, and clay minerals to adsorb rotaries and coronations in vitro. Results revealed that all the adsorbent agents had good to excellent capability of adsorbing rotaries and excellent capability of adsorbing coronations. Percent absorptions ranged from 78.74% to 99.89% for rotaries and 99.99% for coronations; while sand (negative control) was < 0.01%. A high affinity binding was present as determined by a low percent desorption (0.06% to 3.09%). However, the adsorbent bound virus complex retained, and may have actually enhanced, infertility. The second part of this study involves the extraction of crude theaflavin from black tea, which was fractionated by HPLC into five components (initial peaks (IP), TF₁, TF₂[], TF₂[], and TF₃). The crude extract and the various fractions of theaflavin were collected and tested, individually and combined, for antirotaviral activity. The mean effective concentration (EC₅₀) was calculated and compared. Activity varied from the most active being the uncharacterized theaflavin-like initial peaks (IP) with an EC₅₀ of 0.125 []g/ml to the least active being theaflavin-3 monogallate (TF₂[]) with an EC₅₀ of 251.39 []g/ml. The combination of TF₁+TF₂[]+TF₂[]+TF₃ was more active than the sum of the activities of these four fractions individually, indicating synergism among the peaks. Only the crude extract was assayed for activity against coronations; the EC₅₀ was 34.7 []g/ml. |
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| Item Description: | Vita. "Major Subject: Veterinary Microbiology". |
| Physical Description: | ix, 61 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 51-58). |