Construction of a Pseudomonas syringae pv. syringae reporter vector for the study of the expression of the recA gene in response to UV light /
Responsible for such diseases as brown spot of bean, Pseudomonas syringae pv. syringae is an inhabitant on the leaf surface (phyllosphere) of plants and must maintain large population numbers in order to infect host plants. The ability to repair genetic damage caused by UV-B light, that part of the...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
2000.
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| Summary: | Responsible for such diseases as brown spot of bean, Pseudomonas syringae pv. syringae is an inhabitant on the leaf surface (phyllosphere) of plants and must maintain large population numbers in order to infect host plants. The ability to repair genetic damage caused by UV-B light, that part of the solar spectrum from 290-320 nm, is essential to the survival and success of the bacterium. The recA gene is the initiating gene of the regulatory network, known as the SOS repair mechanism, which is responsible for repairing genetic damage blocking DNA replication in many species of bacteria. We have cloned and characterized a putative promoter regio loopbn of the P.s. syringae recA gene by DNA subcloning and sequencing techniques. We constructed a recA::gfp reporter vector using an improved green fluorescent protein cloning cassette shown to be effective in P.s. syringae (Miller and Lindow, 1997). We hope this vector will allow us to quantify the expression of the recA gene in response to UV-B light in two gfp fusion strains of P.s. syringae. The strains containing this plant may be used in several in vitro and environmental studies in order to understand the response of the recA gene and its role within the SOS repair mechanism, and to investigate the habitat of the bacteria within the phyllosphere. |
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| Item Description: | Vita. "Major Subject: Molecular Genetics". |
| Physical Description: | v, 20 leaves : illustrations ; 28 cm. |
| Bibliography: | Includes bibliographical references (leaves 16-19). |