rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Association of intracellular calcium oscillations with release of gonadotropin-releasing hormone (GnRH) from GT1-1 neuronal cells
  name:
    name part: Gilmore, Chrystal Dawn
    name part: 1973-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: xx
    date issued: 1999
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    form: remote
    extent: x, 58 leaves : illustrations ; 28 cm.
  abstract: GnRH is released from its nerve terminals via a calcium (Ca²⁺)-dependent mechanism. Oscillation in the intercellular Ca²⁺ [Ca²⁺][] concentration may encode signals that result in the release of GnRH. The objective of this study was to investigate the relationship of GnRH release with [Ca²⁺][] oscillations using the GT1-1 neuronal cell line. GnRH release was measured by radioimmunoassay of media incubated with GT1-1 neuronal cells for 1 hr with control or test substances to induce release of GnRH. [Ca[]][] oscillations were analyzed by confocal microscopy of fluorescence intensities, at 3 sec intervals, of GT1-1 neuronal cells loaded with fluo-3,AM dye. Depolarization (60 mMK+) induced a consistent 2-fold increase in GnRH release. The response of GT1-1 neuronal cells to treatment with NMDA varied between experiments. Studies with either PGE[] or PGE[] released GnRH in a dose-dependent manner, with PGE[] being more potent than PGE[]. 90% of GT1-1 neuronal cells examined exhibited intrinsic oscillations of [Ca²⁺][]. The frequency or pattern of [Ca²⁺][] oscillations was synchronized within an isolated aggregate of neuronal cells, but varied between unconnected aggregates of neuronal cells. Intrinsic [Ca²⁺][] oscillations disappeared when EGTA was added to the media. Depolarization evoked a transient spike in the [Ca²⁺][] at followed by a sustained elevated plateau of [Ca²⁺][]. NMDA did not significantly alter the frequency of [Ca²⁺][] oscillations. Both PGE[] and PGE[] induced an immediate increase in the frequency of [Ca²⁺][] oscillations. In summary, GT1-1 neuronal cells exhibit intrinsic [Ca²⁺][] oscillations whose frequency or pattern is synchronized between interconnected neuronal cells but vary between unconnected cells. Extracellular Ca²⁺ appears important for [Ca²⁺][] oscillations. Both PGE[] and PGE[], at doses that induce release of GnRH, induce a dramatic increase in the frequency of [Ca²⁺][] at oscillations. These results indicate that release of GnRH is ass…
  note: by Chrystal Dawn Gilmore.
  note: "Major subject: Physiology of Reproduction".
  note: In title numerals are used.
  note: Vita.
  note: Also available online.
  note: Issued also on microfiche from Lange Micrographics.
  note: M.S. Texas A&M University 1999.
  note: Includes bibliographical references (leaves 43-57).
  subject:
    topic: Major physiology of reproduction
  identifier: https://hdl.handle.net/1969.1/ETD-TAMU-1999-THESIS-G652
  related item:
    location:
      url: https://hdl.handle.net/1969.1/ETD-TAMU-1999-THESIS-G652
  record info:
    record content source: TXA
    record creation date: 000905
    record change date: 20220103134236.0
    record identifier: in00001563312