rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Overexpression of metabolic enzymes at the junction of glycolysis and the TCA cycle in Escherichia coli
    sub title: physiological effects and application
  name:
    name part: Spitzer, Richard G.
    name part: 1970-
    role:
      role term: creator
  type of resource: text
  genre: bibliography
  origin info:
    place:
      place term: txu
    date issued: 1999
    issuance: monographic
  language:
    language term: eng
  physical description:
    form: print
    form: remote
    extent: viii, 66 leaves : illustrations ; 28 cm.
  abstract: The metabolism of Escherichia coli has central importance in biochemical engineering, metabolic engineering, and molecular biology. This study relates to all three disciplines. In biochemical engineering, previous studies show that overexpressing the enzyme phosphoenolpyruvate synthase (Pps) has increased the yield of the aromatic precursor 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) from glucose by two- fold compared to previous methods. In this study, Pps overexpression is again applied to DAHP production, but this time, independent modulation of three different overexpression enzymes (DAHP synthase (AroG), transketolase (Tkt), and Pps) was allowed to attempt to optimize DAHP production. In addition to using glucose for this optimization, xylose was also used as a carbon source. With glucose, pathway analysis shows the potential for carbon flux recycle due to Pps because phosphoenolpyruvate (PEP, a precursor for DAHP production) is converted to private during glucose uptake by the phosphotransferase (PTS) system. However, with xylose, pathway analysis showed no theoretical need for Pps overexpression because xylose does not use the PTS system. With xylose, DAHP production is optimized during AroG overexpression alone; Pps helped produce DAHP only slightly when AroG was not overexpressed. The implications of this work to metabolic engineering and molecular biology are the physiological effects of enzyme overexpression on central metabolism. Overexpression of glycolytic phosphoenolpyruvate carboxylase (Ppc) and gluconeogenic phosphoenolpyruvate carboxykinase (Pck) show physiological effects of increased glucose conscription, along with other effects previously observed. When both enzymes are overexpressed together in a futile cycle, more pronounced effects occur including private excretion. Analysis of internal metabolite concentrations shows that fructose 1,6-diphosphate (FDP) concentrations increased for cases increased glucose consumption. This provides so…
  note: by Richard G. Spitzer.
  note: "Major subject: Chemical Engineering".
  note: Vita.
  note: Also available online.
  note: Issued also on microfiche from Lange Micrographics.
  note: M.S. Texas A&M University 1999.
  note: Includes bibliographical references (leaves 58-65).
  subject:
    topic: Major chemical engineering
  identifier: https://hdl.handle.net/1969.1/ETD-TAMU-1999-THESIS-S694
  related item:
    location:
      url: https://hdl.handle.net/1969.1/ETD-TAMU-1999-THESIS-S694
  record info:
    record content source: TXA
    record creation date: 000919
    record change date: 20220103134245.0
    record identifier: in00001562923