Immunocytochemical methods and protocols /
| Other Authors: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, N.J. :
Humana Press,
[1994]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 34. |
| Subjects: |
Table of Contents:
- Antibody preparation
- Overview of antibody use in immunocytochemistry
- Purification of antibodies using ammonium sulfate fractionation or gel filtration
- Purification of antibodies using ion-exchange chromatography
- Purification of antibodies using affinity chromatography
- Purification of antibodies using protein A-sepharose and FPLC
- Conjugation of fluorochromes to antibodies
- Biotinylation of antibodies
- Tissue preparation for light microscopic analyses
- Overview of cell fixation and permeabilization.
- Preparation of frozen sections for analysis
- Processing of cell-touch preparations
- Processing of cytological specimens
- Processing of tissue-culture cells
- Processing of tissue specimens
- Light microscopic detection systems
- Overview of fluorophores
- Direct immunofluorescent labeling of cells
- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells
- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells.
- Fluorescent labeling of surface or intracellular antigens in whole-amounts
- Overview of antigen detection through enzymatic activity
- Peroxidase-antiperoxidase (PAP) method
- Avidin-biotin complex (ABC) method
- Labeled avidin binding (LAB) method
- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue
- ELISA on attachment-dependent or suspension grown cells
- Use of immunogold with silver enhancement
- Fluorescence-activated cell sorter (FACS) analyses
- Overview of flow cytometry and fluorescent probes for cytometry.
- Tissue disaggregation
- Indirect immunofluorescent labeling of viable cells
- Indirect immunofluorescent labeling of fixed cells
- Fluorescent labeling of DNA
- Deparaffinization and processing of pathologic material
- Assay for phagocytosis
- Assay for filamentous actin
- Assay for chemoattractant binding
- Assay for oxidative metabolism
- Colloidal gold detection systems for electron microscopic analyses
- Fixation and embedding
- Preparation of colloidal gold
- Conjugation of colloidal gold to proteins.
- Colloidal gold/streptavidin methods
- Pre-embedding labeling methods
- Postembedding labeling methods
- Photography
- Overview of fluorescence photomicrography
- Special applications
- Overview of fluorescence analysis with the confocal microscope
- Overview of laser microbeam applications as related to antibody targeting
- Nucleic acid immunocytochemistry: in situ hybridization to human chromosomes of an alkaline phosphatase-labeled centromeric probe
- Fluorescence in situ hybridization using whole chromosome library probes.
- Overview of automated immunostainers
- Overview of immunocytochemical approaches to the differential diagnosis of tumors: diagnostic applications in the clinical laboratory.