http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
Modulated expression of actin-cytoskeletal regulatory proteins and endothelial cell differentiation markers during capillary morphogenesis in three-dimensional collagen matrix
viii, 108 leaves : illustrations ; 28 cm.
The formation of capillaries is critical during many physiologic and pathologic processes. The model of capillary morphogenesis in vitro uses human umbilical vein endothelial cells suspended within three-dimensional type I collagen gels. The cells undergo dramatic morphogenic changes to develop capillary lumens, tubes, and networks. Three proteins that regulate the actin cytoskeleton, gelsolin, vasodilator- stimulated phosphoprotein (VASP), and protein, change in expression during the formation of vascular structures. These changes are accompanied by changes in endothelial cell migratory behavior and cell morphology. The endothelial cell is observed to undergo a phenotypic change by examining changes in expression of endothelial marker molecules, adhesion molecules, growth factors, tyrosine kinase receptors, and extracellular matrix molecules. Using adenoviral constructs for VEGF121 and VEGF165, overexpression of these growth factors is observed to affect endothelial cell morphology during capillary morphogenesis. By inhibiting collagen synthesis, the formation of endothelial specific extracellular matrix during capillary morphogenesis is observed to be critical for the formation of vascular structures and maybe for endothelial cell survival in a three-dimensional matrix environment.
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Includes bibliographical references (leaves 74-88).