Molecular genetics of Brassica oleracea and Arabidopsis thaliana /
A detailed comparative map of Brassica oleracea and Arabidopsis thaliana has been established based largely on mapping of Arabidopsis EST: in two Arabidopsis and four Brassica populations. Based on conservative criteria for inferring synteny, ''one to one correspondence'' between...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1999.
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| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=730298121&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | A detailed comparative map of Brassica oleracea and Arabidopsis thaliana has been established based largely on mapping of Arabidopsis EST: in two Arabidopsis and four Brassica populations. Based on conservative criteria for inferring synteny, ''one to one correspondence'' between Brassica and Arabidopsis chromosomes accounted for 57% of comparative loci. Based on 186 corresponding loci detected in #. oleracea and A. thaliana, at least 19 chromosome structural rearrangements differentiate B. oleracea and A. thaliana orthologs. Chromosomal duplication in the A. oleracea genome was strongly suggested by parallel arrangements of duplicated loci on different chromosomes, which accounted for 41% of loci mapped. Based on 367 loci mapped, at least 22 chromosomal rearrangements differentiate B. oleracea homology from one another. Triplication of some Brassica chromatin, and duplication of some Arabidopsis chromatin, was suggested by data that could not be accounted for by the one-to-one, and duplication, models respectively. Twenty-seven probes detected three or more loci in Brassica, which represent 25.3 % of the 367 loci mapped. Thirty-one probes detected two or more loci in Arabidopsis, which represent 23.7 % of the 262 loci mapped. Quantitative trait loci (QTLs) influencing curd size, leaf size and shape, stem architecture and seed yield were detected by restriction fragment length polymorphisms (RFLP) in three Brassica oleracea F2 populations derived from crosses of rapid- cycling-Brassica to three B. oleracea varieties, Cantonese, Pusa Katki and Bugh Kana. By the comparison of QTL maps constructed from three different F2 populations, we identified a total of 201 QTLs, which might reflect variation in as few as 162 different genetic loci, and 129 ancestral genes for 22 traits. Factors that may have contributed to the complex morphology and evolution of the curd are discussed. The Arabidopsis/Brassica system is a useful model for both the challenges and opportunities associated with extrapolation of genomic information from facile models to large- genome taxa including major crops. |
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| Item Description: | Vita. "Major Subject: Genetics". |
| Physical Description: | xi, 157 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 140-153). |