Physical assignment of microsatellites to bovine chromosomes and integration of physical-linkage-RH maps of BTA5 containing comparative genes /
Here we report the physical assignment of 54 microsatellite-containing BACs by FISH to G/Q bands of 14 bovine chromosomes. These represent new assignments that further anchor the linkage maps to specific chromosome regions and allow a more precise estimation of chromosomal coverage. Chromosome recom...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1999.
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| Subjects: | |
| Online Access: | Connect to the full text of this electronic resource |
| Summary: | Here we report the physical assignment of 54 microsatellite-containing BACs by FISH to G/Q bands of 14 bovine chromosomes. These represent new assignments that further anchor the linkage maps to specific chromosome regions and allow a more precise estimation of chromosomal coverage. Chromosome recombination rates were highly variable ranging from 1.38-0.77 Mb/cs: with an average of 0.98 Mb/cM. The regression line showed a decline in the value of the Mb/cM relationship as the chromosome size decreases. Additionally, telomeres of BTA1, BTA2, BTA5 and BTA9 showed higher recombination rates than their controverts. Eighteen BAC clones containing genes, to be used in the constitution of human-bovine comparative maps, were assigned by FISH to BTA5, BTA17 and BTA22. MYF5, SYT1, IGFBP6, HOXC4, SP1, COL2A1, IFNG, LYZ, A2M, IGF1, MB, GAPD, BZRP, DIA1 and CYP2D6 were assigned to different bands on BTA5. IGL@ and PLA2G1B were assigned to BTA17 and ATP6E to BTS22. The assignment of ATP6E to BTA22 represents a novel conserved synteny between this chromosome and HSA22. Human-bovine comparative maps showed presence of multiple rearrangements between both genomes. Thirty-eight markers were scored on the bovine RHA panel. Marker retention percentage per hybrid ranged from 8.9%-25.8% with an average of 18.6%. Pairwise analysis placed 34 markers within a single linkage group with a LOD support of 3.0 and 5 marker pairs showed identical retention patterns. The remaining markers showed no association with any other at this level of support. Maximum likelihood analysis of the 34 markers produced two groups in which order could be established unambiguously. A large linkage group contained 28 markers (11 genes and 17 microsatellites) and spanned 340 cR₅₀₀₀ with an average distance of 12.1 cR₅₀₀₀. The small linkage group contained 3 genes and 2 microsatellites and spanned 66.4 cR₅₀₀₀ with an average distance of 13.3 cR₅₀₀₀. The gap separation between the two ordered groups was estimated to be 78.3 cR₅₀₀₀ which makes the overall map size 484.7 cR[] long. Only GAPD, showing Linkage by pairwise analysis was not ordered and is expected to be within the gap. Twenty-four markers were ordered with a LOD support of at least 2.00. |
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| Item Description: | Vita. "Major Subject: Genetics". In title, numerals and symbols are used. |
| Physical Description: | xiv, 128 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 118-127). |