Cloning and characterization of a pheromone precursor gene of Magnaporthe grisea /
In heterothallic ascomycetes one mating partner serves as the source of female tissue and is fertilized with spermatic from a partner of the opposite mating type. The role of pheromone signaling in mating is thought to involve recognition of cells of the opposite mating type. I have isolated a putat...
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| Format: | Thesis Book |
| Language: | English |
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[Place of publication not identified] :
[publisher not identified] ;
1999.
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| Subjects: | |
| Online Access: | http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=733675261&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD |
| Summary: | In heterothallic ascomycetes one mating partner serves as the source of female tissue and is fertilized with spermatic from a partner of the opposite mating type. The role of pheromone signaling in mating is thought to involve recognition of cells of the opposite mating type. I have isolated a putative pheromone precursor gene from Mat 1-1 strains of M. grisea. The gene, MF1-1, is present in both mating types of the fungus but it is expressed only in the Mat 1-1 strains. The MF1-1 gene is predicted to encode a 26 amino acid polypeptide that is processed to produce a lipopeptide pheromone. The mature pheromone is predicted to contain a C-terminal cysteine residue that is farnesylated and methyl esterified. Expression of this M. grisea putative pheromone precursor gene was observed in defined nutritional conditions and in field isolates. This suggests that the requirement for complex media for mating and the poor fertility of field isolates may not be due to limitation for pheromone precursor gene expression. Detection of putative pheromone precursor gene mRNA in conidia suggests that pheromones may be important for the fertility of conidia acting as spermatic. Characterization of the MF1-1 pheromone gene disruption mutants indicates that pheromones play the roles in male fertility in M grisea. Analysis of the N crassa transformants of the M. grisea MF1-1 pheromone genomic clone suggests that mating type specific regulation of the pheromone precursor gene is conserved in these two fungal species. |
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| Item Description: | Vita. "Major Subject: Plant Pathology". |
| Physical Description: | ix, 86 leaves : illustrations ; 28 cm. Issued also on microfiche from University Microfilm Inc. |
| Bibliography: | Includes bibliographical references (leaves 53-62). |