Gene knockout protocols /
| Other Authors: | , |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press,
[2001]
|
| Series: | Methods in molecular biology (Clifton, N.J.) ;
v. 158. |
| Subjects: |
Table of Contents:
- Overview: gene knockouts
- Isolation and maintenance of primate embryonic stem cells
- Gene targeting in ES cells
- Manipulating mouse embryonic stem cells
- Gene targeting in a centralized facility
- The loxP/CRE system and genome modification
- Creation and use of a Cre recombinase transgenic database
- Choice of mouse strains for gene targeting
- Isolation, microinjection, and transfer of mouse blastocysts
- Aggregatin chimeras: combining ES cells, diploid and tetraploid embryos
- How to study pathologic phenotypes of knockout mice
- Analysis of hematopoietic phenotypes in knockout mouse models
- Isolation of embryonic fibroblasts and their use in the in vitro characterization of gene function
- Influence of genetic background on knockout mouse phenotypes
- Lineage allocation during early embryogenesis: mapping of the neural primordia and application to the analysis of mouse mutants
- Generation of double-knockout, embryonic stem cells
- In vitro differentiation of embryonic stem cells and analysis of cellular phenotypes
- Embryonic stem cells in teh study of hematopoiesis
- Interferon-inducible ES cell expression systems
- Transgenic studies in the mouse: improving the technology towards a conditional temporal and spatial approach
- In vivo libraries of large insert transgenic mice for genetic mapping
- Epigenetic effects on transgene expression
- Positional-candidate cloning of genes from mouse mutants
- Genetically engineered mice: husbandry and resouces
- Embryo cryopreservation for transgenic mouse lines.