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Matrix-assisted laser desorption ionization time-of-flight mass spectrometry : optimizing mass resolution and mass measurement accuracy /

flight (MALDI-TOF) mass spectrometry has rapidly advanced as a method for biomolecule analysis since its introduction almost ten years ago. The great majority of the advances in terms of mass resolution and mass measurement accuracy in this technique have and mass measurement accuracy in this techni...

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Bibliographic Details
Main Author: Edmondson, Ricky Dale
Format: Thesis Book
Language:English
Published: [Place of publication not identified] : [publisher not identified] ; 1998.
Subjects:
Major chemistry.
Online Access:http://proxy.library.tamu.edu/login?url=http://proquest.umi.com/pqdweb?did=733039121&sid=1&Fmt=2&clientId=2945&RQT=309&VName=PQD
Description
Summary:flight (MALDI-TOF) mass spectrometry has rapidly advanced as a method for biomolecule analysis since its introduction almost ten years ago. The great majority of the advances in terms of mass resolution and mass measurement accuracy in this technique have and mass measurement accuracy in this technique have research for this dissertation was performed. The research described herein examines the MALDI ion research described herein examines the MALDI ion observed mass resolution and describes how these limitations can be overcome to yield high resolution MALDI-TOF mass spectra. The instrumental requirements for acquiring high resolution MALDI-TOF mass spectra are then discussed in detail, including the advantages of high resolution mass spectra. One clear advantage of high resolution mass spectra is that it allows the possibility to perform accurate mass measurements. The utility of MALDI-TOF accurate mass measurements in the analysis of biomolecules is discussed herein and focuses on the identification and characterization of proteins using accurate mass MALDI-TOF of enzymatically generated peptide maps.
Item Description:Vita.
"Major Subject: Chemistry".
Physical Description:x, 157 leaves : illustrations ; 28 cm.
Issued also on microfiche from University Microfilm Inc.
Bibliography:Includes bibliographical references (leaves 149-156).